4PIR

X-ray structure of the mouse serotonin 5-HT3 receptor

4PIR の概要

• エントリーDOI: 10.2210/pdb4pir/pdb
• 分子名称: 5-hydroxytryptamine receptor 3A, VHH15, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: membrane transport, ion channel, transport protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 338002.97

構造登録者

Hassaine, G.,Deluz, C.,Grasso, L.,Wyss, R.,Tol, M.B.,Hovius, R.,Graff, A.,Stahlberg, H.,Tomizaki, T.,Desmyter, A.,Moreau, C.,Li, X.-D.,Poitevin, F.,Vogel, H.,Nury, H. (登録日: 2014-05-09, 公開日: 2014-08-06, 最終更新日: 2024-10-30)

主引用文献

Hassaine, G.,Deluz, C.,Grasso, L.,Wyss, R.,Tol, M.B.,Hovius, R.,Graff, A.,Stahlberg, H.,Tomizaki, T.,Desmyter, A.,Moreau, C.,Li, X.D.,Poitevin, F.,Vogel, H.,Nury, H.
X-ray structure of the mouse serotonin 5-HT3 receptor.
Nature, 512:276-281, 2014

PubMed Abstract: Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly understood. Here we present the X-ray structure of a mammalian Cys-loop receptor, the mouse serotonin 5-HT3 receptor, at 3.5 Å resolution. The structure of the proteolysed receptor, made up of two fragments and comprising part of the intracellular domain, was determined in complex with stabilizing nanobodies. The extracellular domain reveals the detailed anatomy of the neurotransmitter binding site capped by a nanobody. The membrane domain delimits an aqueous pore with a 4.6 Å constriction. In the intracellular domain, a bundle of five intracellular helices creates a closed vestibule where lateral portals are obstructed by loops. This 5-HT3 receptor structure, revealing part of the intracellular domain, expands the structural basis for understanding the operating mechanism of mammalian Cys-loop receptors.

PubMed: 25119048
DOI: 10.1038/nature13552

実験手法

X-RAY DIFFRACTION (3.5 Å)