4OIQ

Crystal structure of Thermus thermophilus transcription initiation complex soaked with GE23077 and rifampicin

Summary for 4OIQ

Entry DOI	10.2210/pdb4oiq/pdb
Related	4OIN 4OIO 4OIP 4OIR
Related PRD ID	PRD_001181
Descriptor	DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, (2Z)-2-methylbut-2-enoic acid, ... (12 entities in total)
Functional Keywords	ge23077, transcription inhibitor, transcription open complex, transcription initiation complex, i site, i+1 site, rifampicin, rif, rifampin, rna polymerase, dna/rna/ntp binding, polymerization of ribonucleotides, nucleoid, transcription, transferase-antibiotic complex, transferase/antibiotic
Biological source	Thermus thermophilus More
Cellular location	Cytoplasm (By similarity): Q5SKW1
Total number of polymer chains	9
Total formula weight	444818.42
Authors	Zhang, Y.,Ebright, R.H.,Arnold, E. (deposition date: 2014-01-20, release date: 2014-05-07, Last modification date: 2023-09-20)
Primary citation	Zhang, Y.,Degen, D.,Ho, M.X.,Sineva, E.,Ebright, K.Y.,Ebright, Y.W.,Mekler, V.,Vahedian-Movahed, H.,Feng, Y.,Yin, R.,Tuske, S.,Irschik, H.,Jansen, R.,Maffioli, S.,Donadio, S.,Arnold, E.,Ebright, R.H. GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides. Elife, 3:e02450-e02450, 2014 Cited by PubMed: 24755292 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.624 Å)

Structure validation