4KYW

Restriction endonuclease DPNI in complex with two DNA molecules

Summary for 4KYW

• Entry DOI: 10.2210/pdb4kyw/pdb
• Related: 4ESJ
• Descriptor: Type-2 restriction enzyme DpnI, 5'-(*DC*DTP*DGP*DGP*6MAP*DTP*DCP*DCP*DAP*DG)-3', ZINC ION, ... (6 entities in total)
• Functional Keywords: restriction endonuclease-dna complex, type iim, type iie, restriction enzyme, dpni, methylation dependent, n6-methyladenine, pd-(d/e)xk type endonuclease, winged helix domain, restriction endonuclease, dna binding, hydrolase-dna complex, hydrolase/dna
• Biological source: Streptococcus pneumoniae; More
• Total number of polymer chains: 5
• Total formula weight: 42442.96

Authors

Mierzejewska, K.,Siwek, W.,Czapinska, H.,Skowronek, K.,Bujnicki, J.M.,Bochtler, M. (deposition date: 2013-05-29, release date: 2014-06-04, Last modification date: 2023-09-20)

• Primary citation: Mierzejewska, K.,Siwek, W.,Czapinska, H.,Kaus-Drobek, M.,Radlinska, M.,Skowronek, K.,Bujnicki, J.M.,Dadlez, M.,Bochtler, M.; Structural basis of the methylation specificity of R.DpnI.; Nucleic Acids Res., 42:8745-8754, 2014; PubMed: 24966351; DOI: 10.1093/nar/gku546

Experimental method

X-RAY DIFFRACTION (2.35 Å)