3WZN

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin at 1.3 A resolution

Summary for 3WZN

• Entry DOI: 10.2210/pdb3wzn/pdb
• Related: 3WZO 3WZP 3WZQ
• Descriptor: Streptavidin, BIOTIN, SULFATE ION, ... (4 entities in total)
• Functional Keywords: beta-barrel, biotin binding protein
• Biological source: Streptomyces avidinii
• Cellular location: Secreted: P22629
• Total number of polymer chains: 2
• Total formula weight: 27039.16

Authors

Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Tsumoto, K.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. (deposition date: 2014-10-01, release date: 2015-02-18, Last modification date: 2023-11-08)

• Primary citation: Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A.; Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.; J.Biochem., 157:467-475, 2015; PubMed: 25645976; DOI: 10.1093/jb/mvv004

Experimental method

X-RAY DIFFRACTION (1.3 Å)