3S32

Crystal structure of Ash2L N-terminal domain

Summary for 3S32

• Entry DOI: 10.2210/pdb3s32/pdb
• Descriptor: Set1/Ash2 histone methyltransferase complex subunit ASH2, ZINC ION (3 entities in total)
• Functional Keywords: histone, chromatin, trithorax, helix-wing-helix, c4-zinc finger, trithorax group protein, myeloid lymphoma leukemia protein, nucleus, transcription
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus (Probable): Q9UBL3
• Total number of polymer chains: 1
• Total formula weight: 22104.85

Authors

Sarvan, S.,Avdic, V.,Tremblay, V.,Chaturvedi, C.-P.,Zhang, P.,Lanouette, S.,Blais, A.,Brunzelle, J.S.,Brand, M.,Couture, J.-F. (deposition date: 2011-05-17, release date: 2011-06-08, Last modification date: 2024-11-06)

Primary citation

Sarvan, S.,Avdic, V.,Tremblay, V.,Chaturvedi, C.P.,Zhang, P.,Lanouette, S.,Blais, A.,Brunzelle, J.S.,Brand, M.,Couture, J.F.
Crystal structure of the trithorax group protein ASH2L reveals a forkhead-like DNA binding domain.
Nat.Struct.Mol.Biol., 18:857-859, 2011

PubMed Abstract: Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the β-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the β-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain.

PubMed: 21642971
DOI: 10.1038/nsmb.2093

Experimental method

X-RAY DIFFRACTION (2.45 Å)