3PZD

Structure of the myosin X MyTH4-FERM/DCC complex

Summary for 3PZD

• Entry DOI: 10.2210/pdb3pzd/pdb
• Descriptor: Myosin-X, Netrin receptor DCC, GLYCEROL, ... (4 entities in total)
• Functional Keywords: protein-protein complex, myth4 domain, ferm domain, cargo binding, motor protein-apoptosis complex, motor protein/apoptosis
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm, cytosol: Q9HD67; Membrane; Single-pass type I membrane protein: P70211
• Total number of polymer chains: 2
• Total formula weight: 62700.61

Authors

Wei, Z.,Yan, J.,Pan, L.,Zhang, M. (deposition date: 2010-12-14, release date: 2011-02-23, Last modification date: 2024-03-20)

Primary citation

Wei, Z.,Yan, J.,Lu, Q.,Pan, L.,Zhang, M.
Cargo recognition mechanism of myosin X revealed by the structure of its tail MyTH4-FERM tandem in complex with the DCC P3 domain
Proc.Natl.Acad.Sci.USA, 108:3572-3577, 2011

PubMed Abstract: Myosin X (MyoX), encoded by Myo10, is a representative member of the MyTH4-FERM domain-containing myosins, and this family of unconventional myosins shares common functions in promoting formation of filopodia/stereocilia structures in many cell types with unknown mechanisms. Here, we present the structure of the MyoX MyTH4-FERM tandem in complex with the cytoplasmic tail P3 domain of the netrin receptor DCC. The structure, together with biochemical studies, reveals that the MyoX MyTH4 and FERM domains interact with each other, forming a structural and functional supramodule. Instead of forming an extended β-strand structure in other FERM binding targets, DCC_P3 forms a single α-helix and binds to the αβ-groove formed by β5 and α1 of the MyoX FERM F3 lobe. Structure-based amino acid sequence analysis reveals that the key polar residues forming the inter-MyTH4/FERM interface are absolutely conserved in all MyTH4-FERM tandem-containing proteins, suggesting that the supramodular nature of the MyTH4-FERM tandem is likely a general property for all MyTH4-FERM proteins.

PubMed: 21321230
DOI: 10.1073/pnas.1016567108

Experimental method

X-RAY DIFFRACTION (2.5 Å)