3I5K

Crystal structure of the NS5B polymerase from Hepatitis C Virus (HCV) strain JFH1

Summary for 3I5K

• Entry DOI: 10.2210/pdb3i5k/pdb
• Descriptor: RNA-directed RNA polymerase, PHOSPHATE ION (3 entities in total)
• Functional Keywords: rdrp structure (fingers, palm, thumb domains), apoptosis, atp-binding, capsid protein, cell membrane, disulfide bond, endoplasmic reticulum, envelope protein, fusion protein, glycoprotein, helicase, host-virus interaction, hydrolase, interferon antiviral system evasion, lipid droplet, lipoprotein, membrane, metal-binding, mitochondrion, multifunctional enzyme, nucleotide-binding, nucleotidyltransferase, nucleus, oncogene, palmitate, phosphoprotein, protease, ribonucleoprotein, rna replication, rna-binding, rna-directed rna polymerase, secreted, serine protease, sh3-binding, thiol protease, transcription, transcription regulation, transferase, transmembrane, viral nucleoprotein, virion
• Biological source: Hepatitis C virus JFH-1 (HCV)
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Core protein p19: Virion (By similarity). Envelope glycoprotein E1: Virion membrane; Single-pass type I membrane protein (Potential). Envelope glycoprotein E2: Virion membrane; Single-pass type I membrane protein (Potential). p7: Host endoplasmic reticulum membrane; Multi-pass membrane protein. Protease NS2-3: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein (Probable). Non-structural protein 4A: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 5A: Host endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). RNA-directed RNA polymerase: Host endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential): Q99IB8
• Total number of polymer chains: 4
• Total formula weight: 253503.54

Authors

Simister, P.C.,Schmitt, M.,Lohmann, V.,Bressanelli, S. (deposition date: 2009-07-05, release date: 2009-09-22, Last modification date: 2024-03-20)

• Primary citation: Simister, P.,Schmitt, M.,Geitmann, M.,Wicht, O.,Danielson, U.H.,Klein, R.,Bressanelli, S.,Lohmann, V.; Structural and functional analysis of hepatitis C virus strain JFH1 polymerase; J.Virol., 83:11926-11939, 2009; PubMed: 19740982; DOI: 10.1128/JVI.01008-09

Experimental method

X-RAY DIFFRACTION (2.2 Å)