3H65

The Crystal Structure of C176A Mutated [Fe]-Hydrogenase (Hmd) Holoenzyme in Complex with Methylenetetrahydromethanopterin

Summary for 3H65

Entry DOI	10.2210/pdb3h65/pdb
Related	2B0J 3F46 3F47
Descriptor	5,10-methenyltetrahydromethanopterin hydrogenase, 5'-O-[(S)-hydroxy{[2-hydroxy-3,5-dimethyl-6-(2-oxoethyl)pyridin-4-yl]oxy}phosphoryl]guanosine, FE (II) ION, ... (7 entities in total)
Functional Keywords	rossmann fold, helix bundle, complex with iron guanylyl pyridinol cofactor, c176a mutant, complex with methylenetetrahydromethanopterin, methanogenesis, one-carbon metabolism, oxidoreductase
Biological source	Methanocaldococcus jannaschii (Methanococcus jannaschii)
Total number of polymer chains	1
Total formula weight	40288.21
Authors	Hiromoto, T.,Warkentin, E.,Shima, S.,Ermler, U. (deposition date: 2009-04-23, release date: 2009-09-01, Last modification date: 2023-11-01)
Primary citation	Hiromoto, T.,Warkentin, E.,Moll, J.,Ermler, U.,Shima, S. The crystal structure of an [Fe]-hydrogenase-substrate complex reveals the framework for H2 activation. Angew.Chem.Int.Ed.Engl., 48:6457-6460, 2009 Cited by PubMed: 19623593 DOI: 10.1002/anie.200902695 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.15 Å)

Structure validation