3G7C

Structure of the Phosphorylation Mimetic of Occludin C-term Tail

3G7C の概要

• エントリーDOI: 10.2210/pdb3g7c/pdb
• 関連するPDBエントリー: 1WPA 1XAW
• 分子名称: Occludin (2 entities in total)
• 機能のキーワード: occludin, diabetic retinopathy, zo-1, tight junction, adhesion, cell junction, coiled coil, membrane, phosphoprotein, transmembrane, cell adhesion
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13636.14

構造登録者

Tash, B.R.,Sundstrom, J.M.,Murakami, T.,Flanagan, J.M.,Bewley, M.C.,Antonetii, D.A. (登録日: 2009-02-09, 公開日: 2009-05-12, 最終更新日: 2023-09-06)

主引用文献

Sundstrom, J.M.,Tash, B.R.,Murakami, T.,Flanagan, J.M.,Bewley, M.C.,Stanley, B.A.,Gonsar, K.B.,Antonetti, D.A.
Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach.
J.PROTEOME RES., 8:808-817, 2009

PubMed Abstract: The molecular function of occludin, an integral membrane component of tight junctions, remains unclear. VEGF-induced phosphorylation sites were mapped on occludin by combining MS data analysis with bioinformatics. In vivo phosphorylation of Ser490 was validated and protein interaction studies combined with crystal structure analysis suggest that Ser490 phosphorylation attenuates the interaction between occludin and ZO-1. This study demonstrates that combining MS data and bioinformatics can successfully identify novel phosphorylation sites from limiting samples.

PubMed: 19125584
DOI: 10.1021/pr7007913

実験手法

X-RAY DIFFRACTION (2 Å)