2WLQ

Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation

Summary for 2WLQ

• Entry DOI: 10.2210/pdb2wlq/pdb
• Related: 2CL2 2W39 2W52 2WNE
• Descriptor: PUTATIVE LAMINARINASE, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: laminarin, family 16, cyclical poysaccharides, glycosyl hydrolase, beta sandwich, basidiomycete, beta-glucanase, gh7, gh16, lam16a, beta-1\, 6-glucan, hydrolase
• Biological source: PHANEROCHAETE CHRYSOSPORIUM
• Total number of polymer chains: 1
• Total formula weight: 34268.79

Authors

Vasur, J.,Kawai, R.,Andersson, E.,Widmalm, G.,Jonsson, K.H.,Hansson, H.,Engstrom, A.,Igarashi, K.,Sandgren, M.,Samejima, M.,Stahlberg, J. (deposition date: 2009-06-24, release date: 2010-01-26, Last modification date: 2023-12-13)

• Primary citation: Vasur, J.,Kawai, R.,Jonsson, K.H.,Widmalm, G.,Engstrom, A.,Frank, M.,Andersson, E.,Hansson, H.,Forsberg, Z.,Igarashi, K.,Samejima, M.,Sandgren, M.,Stahlberg, J.; Synthesis of Cyclic Beta-Glucan Using Laminarinase 16A Glycosynthase Mutant from the Basidiomycete Phanerochaete Chrysosporium.; J.Am.Chem.Soc., 132:1724-, 2010; PubMed: 20078120; DOI: 10.1021/JA909129B

Experimental method

X-RAY DIFFRACTION (1.4 Å)