2GW3

Crystal structure of stony coral fluorescent protein Kaede, green form

2GW3 の概要

• エントリーDOI: 10.2210/pdb2gw3/pdb
• 関連するPDBエントリー: 2GW4
• 分子名称: Kaede, NICKEL (II) ION (3 entities in total)
• 機能のキーワード: beta barrel, luminescent protein
• 由来する生物種: Trachyphyllia geoffroyi
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51948.06

構造登録者

Hayashi, I.,Mizuno, H.,Miyawaki, A.,Ikura, M. (登録日: 2006-05-03, 公開日: 2007-05-08, 最終更新日: 2024-10-30)

主引用文献

Hayashi, I.,Mizuno, H.,Tong, K.I.,Furuta, T.,Tanaka, F.,Yoshimura, M.,Miyawaki, A.,Ikura, M.
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.
J.Mol.Biol., 372:918-926, 2007

PubMed Abstract: A coral fluorescent protein from Trachyphyllia geoffroyi, Kaede, possesses a tripeptide of His62-Tyr63-Gly64, which forms a chromophore with green fluorescence. This chromophore's fluorescence turns red following UV light irradiation. We have previously shown that such photoconversion is achieved by a formal beta-elimination reaction, which results in a cleavage of the peptide bond found between the amide nitrogen and the alpha-carbon at His62. However, the stereochemical arrangement of the chromophore and the precise structural basis for this reaction mechanism previously remained unknown. Here, we report the crystal structures of the green and red form of Kaede at 1.4 A and 1.6 A resolutions, respectively. Our structures depict the cleaved peptide bond in the red form. The chromophore conformations both in the green and red forms are similar, except a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule.

PubMed: 17692334
DOI: 10.1016/j.jmb.2007.06.037

実験手法

X-RAY DIFFRACTION (1.4 Å)