1SZ9
The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model
Summary for 1SZ9
| Entry DOI | 10.2210/pdb1sz9/pdb |
| Related | 1SZA |
| Descriptor | PCF11 protein (2 entities in total) |
| Functional Keywords | pcf11, rna polymerase ii ctd interacting domain, arm repeats, transcription |
| Biological source | Saccharomyces cerevisiae (baker's yeast) |
| Cellular location | Nucleus : P39081 |
| Total number of polymer chains | 3 |
| Total formula weight | 49872.43 |
| Authors | Meinhart, A.,Cramer, P. (deposition date: 2004-04-05, release date: 2004-07-13, Last modification date: 2024-04-03) |
| Primary citation | Meinhart, A.,Cramer, P. Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors. Nature, 430:223-226, 2004 Cited by PubMed Abstract: During transcription, RNA polymerase (Pol) II synthesizes eukaryotic messenger RNA. Transcription is coupled to RNA processing by the carboxy-terminal domain (CTD) of Pol II, which consists of up to 52 repeats of the sequence Tyr 1-Ser 2-Pro 3-Thr 4-Ser 5-Pro 6-Ser 7 (refs 1, 2). After phosphorylation, the CTD binds tightly to a conserved CTD-interacting domain (CID) present in the proteins Pcf11 and Nrd1, which are essential and evolutionarily conserved factors for polyadenylation-dependent and -independent 3'-RNA processing, respectively. Here we describe the structure of a Ser 2-phosphorylated CTD peptide bound to the CID domain of Pcf11. The CTD motif Ser 2-Pro 3-Thr 4-Ser 5 forms a beta-turn that binds to a conserved groove in the CID domain. The Ser 2 phosphate group does not make direct contact with the CID domain, but may be recognized indirectly because it stabilizes the beta-turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta-spiral model of the CTD. The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner. PubMed: 15241417DOI: 10.1038/nature02679 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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