1I2H

CRYSTAL STRUCTURE ANALYSIS OF PSD-ZIP45(HOMER1C/VESL-1L)CONSERVED HOMER 1 DOMAIN

1I2H の概要

• エントリーDOI: 10.2210/pdb1i2h/pdb
• 分子名称: PSD-ZIP45(HOMER-1C/VESL-1L) (2 entities in total)
• 機能のキーワード: enabled vasp homology 1 domain, signaling protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18823.73

構造登録者

Irie, K.,Nakatsu, T.,Mitsuoka, K.,Fujiyoshi, Y.,Kato, H. (登録日: 2001-02-09, 公開日: 2002-05-29, 最終更新日: 2023-08-09)

主引用文献

Irie, K.,Nakatsu, T.,Mitsuoka, K.,Miyazawa, A.,Sobue, K.,Hiroaki, Y.,Doi, T.,Fujiyoshi, Y.,Kato, H.
Crystal Structure of the Homer 1 Family Conserved Region Reveals the Interaction Between the EVH1 Domain and Own Proline-rich Motif
J.Mol.Biol., 318:1117-1126, 2002

PubMed Abstract: PSD-Zip45 (also named Homer 1c/Vesl-1L) is a synaptic scaffolding protein, which interacts with neurotransmitter receptors and other scaffolding proteins to target them into post-synaptic density (PSD), a specialized protein complex at the synaptic junction. Binding of the PSD-Zip45 to the receptors and scaffolding proteins results in colocalization and clustering of its binding partners in PSD. It has an Ena/VASP homology 1 (EVH1) domain in the N terminus for receptor binding, two leucine zipper motifs in the C terminus for clustering, and a linking region whose function is unclear despite the high level of conservation within the Homer 1 family. The X-ray crystallographic analysis of the largest fragment of residues 1-163, including an EVH1 domain reported here, demonstrates that the EVH1 domain contains an alpha-helix longer than that of the previous models, and that the linking part included in the conserved region of Homer 1 (CRH1) of the PSD-Zip45 interacts with the EVH1 domain of the neighbour CRH1 molecule in the crystal. The results suggest that the EVH1 domain recognizes the PPXXF motif found in the binding partners, and the SPLTP sequence (P-motif) in the linking region of the CRH1. The two types of binding are partly overlapped in the EVH1 domain, implying a mechanism to regulate multimerization of Homer 1 family proteins.

PubMed: 12054806
DOI: 10.1016/S0022-2836(02)00170-5

実験手法

X-RAY DIFFRACTION (1.8 Å)