1T9I

I-CreI(D20N)/DNA complex

Summary for 1T9I

• Entry DOI: 10.2210/pdb1t9i/pdb
• Related: 1G9Y 1G9Z 1T9J
• Descriptor: 5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3', 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3', DNA endonuclease I-CreI, ... (6 entities in total)
• Functional Keywords: protein, dna, hydrolase-dna complex, hydrolase/dna
• Biological source: Chlamydomonas reinhardtii
• Cellular location: Plastid, chloroplast: P05725
• Total number of polymer chains: 4
• Total formula weight: 52354.79

Authors

Chevalier, B.,Sussman, D.,Otis, C.,Boudreau, D.,Turmel, M.,Lemieux, C.,Stephens, K.,Monnat Jr., R.J.,Stoddard, B.L. (deposition date: 2004-05-17, release date: 2004-11-16, Last modification date: 2023-08-23)

• Primary citation: Chevalier, B.,Sussman, D.,Otis, C.,Noel, A.J.,Turmel, M.,Lemieux, C.,Stephens, K.,Monnat Jr., R.J.,Stoddard, B.L.; Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease.; Biochemistry, 43:14015-14026, 2004; PubMed: 15518550; DOI: 10.1021/bi048970c

Experimental method

X-RAY DIFFRACTION (1.6 Å)