6E13
Pseudomonas putida PqqB with a non-physiological zinc at the active site binds the substrate mimic, 5-cysteinyl-3,4-dihydroxyphenylalanine (5-Cys-DOPA), non-specifically but supports the proposed function of the enzyme in pyrroloquinoline quinone biosynthesis.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Coenzyme PQQ synthesis protein B | polymer | 308 | 33999.5 | 1 | UniProt (Q88QV5) Pfam (PF12706) In PDB | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) | Pyrroloquinoline quinone biosynthesis protein B |
| 2 | A | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 3 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | A | 3-{[(2S)-2-amino-2-carboxyethyl]sulfanyl}-5-hydroxy-L-tyrosine | non-polymer | 316.3 | 1 | Chemie (HKS) | |||
| 5 | water | water | 18.0 | 31 | Chemie (HOH) |
Sequence modifications
A: 1 - 303 (UniProt: Q88QV5)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ala 11 | Gly 11 | conflict |
| Thr 253 | Met 253 | conflict |
| Leu 304 | - | expression tag |
| Glu 305 | - | expression tag |
| His 306 | - | expression tag |
| His 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33999.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 482.6 | |
| All* | Total formula weight | 34482.1 |
