6D83

Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant dimer monomeric subunit

エンティティ

Entity ID	鎖名	説明	種類	鎖長	化学式量	分子数	データベース名(アクセス番号)	由来する生物種	エンティティの一般名
1	T, L	Bone marrow stromal antigen 2,Protein Nef	polymer	264	29880.2	2	UniProt (Q10589) UniProt (Q90VU7) Pfam (PF00469) In PDB	Homo sapiens (Human)	BST-2,HM1.24 antigen,Tetherin,3'ORF,Negative factor,F-protein
2	B	AP-1 complex subunit beta-1	polymer	586	66152.5	1	UniProt (Q10567) Pfam (PF01602) In PDB	Homo sapiens (Human)	Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit
3	C, H	ADP-ribosylation factor 1	polymer	193	22314.3	2	UniProt (P84077) Pfam (PF00025) In PDB	Homo sapiens (Human)
4	G	AP-1 complex subunit gamma-1	polymer	601	68194.1	1	UniProt (P22892) Pfam (PF01602) In PDB	Mus musculus (Mouse)	Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1
5	M	AP-1 complex subunit mu-1	polymer	423	48606.7	1	UniProt (P35585) Pfam (PF01217) Pfam (PF00928) In PDB	Mus musculus (Mouse)	AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin
6	S	AP-1 complex subunit sigma-3	polymer	154	18321.3	1	UniProt (Q96PC3) Pfam (PF01217) In PDB	Homo sapiens (Human)	Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin
7	C, H	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
8	C, H	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

配列変更

T, L: 2 - 21 (UniProt: Q10589)

PDB	外部データベース	詳細
Met -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gly 22	-	linker
Ser 23	-	linker
Asp 24	-	linker
Glu 25	-	linker
Ala 26	-	linker
Ser 27	-	linker
Glu 28	-	linker
Gly 29	-	linker
Ser 30	-	linker
Gly 31	-	linker

T, L: 32 - 237 (UniProt: Q90VU7)

PDB	外部データベース	詳細
Ala 195	Leu 164	engineered mutation
Ala 196	Leu 165	engineered mutation

B: 1 - 584 (UniProt: Q10567)

PDB	外部データベース	詳細
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 359	Lys 359	conflict
Lys 476	Glu 476	conflict

C, H: 17 - 181 (UniProt: P84077)

PDB	外部データベース	詳細
Met -11	-	expression tag
Ser -10	-	expression tag
Tyr -9	-	expression tag
Tyr -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Asp -1	-	expression tag
Tyr 0	-	expression tag
Asp 1	-	expression tag
Ile 2	-	expression tag
Pro 3	-	expression tag
Thr 4	-	expression tag
Thr 5	-	expression tag
Glu 6	-	expression tag
Asn 7	-	expression tag
Leu 8	-	expression tag
Tyr 9	-	expression tag
Phe 10	-	expression tag
Gln 11	-	expression tag
Gly 12	-	expression tag
Ala 13	-	expression tag
Met 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Leu 71	Gln 71	conflict

G: 1 - 595 (UniProt: P22892)

PDB	外部データベース	詳細
Glu 596	-	expression tag
Asn 597	-	expression tag
Leu 598	-	expression tag
Tyr 599	-	expression tag
Phe 600	-	expression tag
Gln 601	-	expression tag

S: 1 - 154 (UniProt: Q96PC3)

PDB	外部データベース	詳細
Cys 148	Ser 148	conflict

非対称単位の内容

タンパク質・ 核酸鎖	鎖の数	8
	化学式量合計	305663.5
タンパク質・ 核酸・ 糖鎖以外*	分子数	4
	化学式量合計	1095.0
全て*	化学式量合計	306758.5

*水分子は含んでいません