6C04

Mtb RNAP Holo/RbpA/double fork DNA -closed clamp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (A0A045J8T1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P9WGZ1) In PDB	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1179	130070.8	1	UniProt (V9Z879) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P9WGY9) In PDB	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1326	148202.2	1	UniProt (A0A045J9E2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P9WGY7) In PDB	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	110	11777.0	1	UniProt (A0A0T9N9K3) Pfam (PF01192) UniProt (by SIFTS) (P9WGY5) In PDB	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor SigA	polymer	531	58169.5	1	UniProt (A0A045HD00) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P9WGI1) In PDB	Mycobacterium tuberculosis
6	J	RNA polymerase-binding protein RbpA	polymer	111	12993.7	1	UniProt (A0A045IP01) Pfam (PF13397) UniProt (by SIFTS) (P9WHJ5) In PDB	Mycobacterium tuberculosis
7	O, H	DNA (31-MER)	polymer	31	9565.2	2		Mycobacterium tuberculosis
8	P, G	DNA (26-MER)	polymer	26	7930.2	2		Mycobacterium tuberculosis
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

C: 7 - 1178 (UniProt: V9Z879)

PDB	External Database	Details
Leu 1179	-	expression tag
Ala 1180	-	expression tag
Arg 1181	-	expression tag
His 1182	-	expression tag
Gly 1183	-	expression tag
Gly 1184	-	expression tag
Ser 1185	-	expression tag

D: 1 - 1316 (UniProt: A0A045J9E2)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag

E: 2 - 110 (UniProt: A0A0T9N9K3)

PDB	External Database	Details
Gly 1	-	expression tag

F: 1 - 528 (UniProt: A0A045HD00)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	471694.5
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	471849.7

*Water molecules are not included.