5O89

Crystal Structure of rsEGFP2 in the fluorescent on-state determined by SFX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	248	28099.7	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)	rsEGFP2
2		water	water		18.0	309	Chemie (HOH)

Sequence modifications

A: 3 - 239 (UniProt: P42212)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Thr -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Leu 65	Phe 64	engineered mutation
Pia 68	Ser 65	chromophore
Pia 68	Tyr 66	chromophore
Pia 68	Gly 67	chromophore
Leu 70	Gln 69	engineered mutation
Ser 164	Val 163	engineered mutation
Lys 207	Ala 206	engineered mutation
Leu 232	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28099.7
All*	Total formula weight	28099.7

*Water molecules are not included.