Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5LZV

Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	uL2	polymer	257	28088.9	1	UniProt (G1TT27) Pfam (PF00181) Pfam (PF03947) In PDB	Oryctolagus cuniculus (Rabbit)
2	B	uL3	polymer	403	46108.0	1	UniProt (G1TL06) Pfam (PF00297) In PDB	Oryctolagus cuniculus (Rabbit)
3	C	uL4	polymer	425	47727.6	1	UniProt (G1SVW5) Pfam (PF00573) Pfam (PF14374) In PDB	Oryctolagus cuniculus (Rabbit)
4	D	uL18	polymer	297	34481.8	1	UniProt (G1SYJ6) Pfam (PF17144) Pfam (PF14204) In PDB	Oryctolagus cuniculus (Rabbit)
5	E	eL6	polymer	291	33028.3	1	UniProt (G1SKF7) Pfam (PF03868) Pfam (PF01159) In PDB	Oryctolagus cuniculus (Rabbit)
6	F	uL30	polymer	247	29201.8	1	UniProt (G1TUB1) Pfam (PF08079) Pfam (PF00327) In PDB	Oryctolagus cuniculus (Rabbit)
7	G	eL8	polymer	319	36221.5	1	UniProt (G1STW0) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
8	H	uL6	polymer	192	21871.4	1	UniProt (G1TX33) Pfam (PF00347) UniProt (by SIFTS) (G1SWI6) In PDB	Oryctolagus cuniculus (Rabbit)
9	I	uL16	polymer	214	24643.1	1	UniProt (B7NZQ2) Pfam (PF00252) In PDB	Oryctolagus cuniculus (Rabbit)
10	J	uL5	polymer	178	20288.5	1	UniProt (G1TUB8) Pfam (PF00281) Pfam (PF00673) In PDB	Oryctolagus cuniculus (Rabbit)
11	L	eL13	polymer	211	24331.7	1		Oryctolagus cuniculus (Rabbit)
12	M	eL14	polymer	218	23870.5	1	UniProt (G1SZ12) Pfam (PF01929) In PDB	Oryctolagus cuniculus (Rabbit)
13	N	eL15	polymer	204	24207.3	1	UniProt (G1T0C1) Pfam (PF00827) In PDB	Oryctolagus cuniculus (Rabbit)
14	O	uL13	polymer	203	23533.3	1		Oryctolagus cuniculus (Rabbit)
15	P	uL22	polymer	184	21444.2	1	UniProt (G1TVT6) Pfam (PF00237) In PDB	Oryctolagus cuniculus (Rabbit)
16	Q	eL18	polymer	188	21699.7	1		Oryctolagus cuniculus (Rabbit)
17	R	eL19	polymer	196	23535.3	1		Oryctolagus cuniculus (Rabbit)
18	S	eL20	polymer	176	20827.6	1		Oryctolagus cuniculus (Rabbit)
19	T	eL21	polymer	160	18610.0	1	UniProt (G1SHQ2) Pfam (PF01157) In PDB	Oryctolagus cuniculus (Rabbit)
20	U	eL22	polymer	128	14813.0	1		Oryctolagus cuniculus (Rabbit)
21	V	uL14	polymer	140	14892.5	1	UniProt (G1T6D1) Pfam (PF00238) In PDB	Oryctolagus cuniculus (Rabbit)
22	W	eL24	polymer	157	17825.1	1	UniProt (G1SE28) Pfam (PF01246) In PDB	Oryctolagus cuniculus (Rabbit)
23	X	uL23	polymer	156	17768.2	1	UniProt (G1SE76) Pfam (PF03939) Pfam (PF00276) In PDB	Oryctolagus cuniculus (Rabbit)
24	Y	uL24	polymer	145	17303.4	1	UniProt (G1SQH0) Pfam (PF16906) Pfam (PF00467) In PDB	Oryctolagus cuniculus (Rabbit)
25	Z	eL27	polymer	136	15835.8	1	UniProt (G1TXF6) Pfam (PF00467) Pfam (PF01777) In PDB	Oryctolagus cuniculus (Rabbit)
26	a	uL15	polymer	148	16620.6	1	UniProt (G1SNY0) Pfam (PF00828) In PDB	Oryctolagus cuniculus (Rabbit)
27	b	eL29	polymer	245	26708.7	1	UniProt (G1SGR6) Pfam (PF01779) In PDB	Oryctolagus cuniculus (Rabbit)
28	c	eL30	polymer	115	12807.1	1	UniProt (G1TDL2) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
29	d	eL31	polymer	125	14494.9	1	UniProt (G1SHG0) Pfam (PF01198) In PDB	Oryctolagus cuniculus (Rabbit)
30	e	eL32	polymer	135	15898.9	1	UniProt (G1U437) Pfam (PF01655) UniProt (by SIFTS) (G1TUN8) In PDB	Oryctolagus cuniculus (Rabbit)
31	f	eL33	polymer	110	12580.8	1	UniProt (G1SF08) Pfam (PF01247) In PDB	Oryctolagus cuniculus (Rabbit)
32	g	eL34	polymer	117	13326.1	1	UniProt (G1U945) Pfam (PF01199) In PDB	Oryctolagus cuniculus (Rabbit)
33	h	uL29	polymer	123	14566.6	1	UniProt (G1SIT5) Pfam (PF00831) In PDB	Oryctolagus cuniculus (Rabbit)
34	i	eL36	polymer	105	12263.8	1	UniProt (G1TTQ5) Pfam (PF01158) In PDB	Oryctolagus cuniculus (Rabbit)
35	j	eL37	polymer	97	11111.0	1	UniProt (U3KPD5) Pfam (PF01907) In PDB	Oryctolagus cuniculus (Rabbit)
36	k	eL38	polymer	70	8238.9	1		Oryctolagus cuniculus (Rabbit)
37	l	eL39	polymer	51	6455.8	1	UniProt (G1TTN1) Pfam (PF00832) In PDB	Oryctolagus cuniculus (Rabbit)
38	m	eL40	polymer	102	11699.8	1		Oryctolagus cuniculus (Rabbit)
39	n	eL41	polymer	25	3473.5	1		Oryctolagus cuniculus (Rabbit)
40	o	eL42	polymer	106	12477.0	1	UniProt (G1U344) Pfam (PF00935) UniProt (by SIFTS) (G1T040) In PDB	Oryctolagus cuniculus (Rabbit)
41	p	eL43	polymer	92	10299.4	1	UniProt (G1SY53) Pfam (PF01780) In PDB	Oryctolagus cuniculus (Rabbit)
42	r	eL28	polymer	137	15783.6	1	UniProt (G1U7L1) Pfam (PF01778) In PDB	Oryctolagus cuniculus (Rabbit)
43	s	uL10	polymer	318	34380.5	1		Oryctolagus cuniculus (Rabbit)
44	t	uL11	polymer	165	17847.6	1	UniProt (G1SMR7) Pfam (PF03946) Pfam (PF00298) In PDB	Oryctolagus cuniculus (Rabbit)
45	1	Nascent chain	polymer	15	1788.0	1		Oryctolagus cuniculus (Rabbit)
46	2	P-site tRNA	polymer	76	24436.6	1		Oryctolagus cuniculus (Rabbit)
47	3	E-site tRNA	polymer	75	24102.3	1		Oryctolagus cuniculus (Rabbit)
48	5	28S ribosomal RNA	polymer	3543	1148115.4	1		Oryctolagus cuniculus (Rabbit)
49	7	5S ribosomal RNA	polymer	120	38691.9	1		Oryctolagus cuniculus (Rabbit)
50	8	5.8S ribosomal RNA	polymer	156	50143.6	1		Oryctolagus cuniculus (Rabbit)
51	9	18S ribosomal RNA	polymer	1869	602778.9	1		Oryctolagus cuniculus (Rabbit)
52	AA	uS2	polymer	295	32958.0	1		Oryctolagus cuniculus (Rabbit)
53	BB	eS1	polymer	264	30002.1	1	UniProt (G1SS70) Pfam (PF01015) In PDB	Oryctolagus cuniculus (Rabbit)
54	CC	uS5	polymer	293	31327.4	1		Oryctolagus cuniculus (Rabbit)
55	DD	uS3	polymer	243	26715.3	1	UniProt (G1TNM3) Pfam (PF00189) Pfam (PF07650) In PDB	Oryctolagus cuniculus (Rabbit)
56	EE	eS4	polymer	263	29654.9	1		Oryctolagus cuniculus (Rabbit)
57	FF	uS7	polymer	204	22913.5	1	UniProt (G1TFM5) Pfam (PF00177) In PDB	Oryctolagus cuniculus (Rabbit)
58	GG	eS6	polymer	249	28751.9	1	UniProt (G1TM55) Pfam (PF01092) In PDB	Oryctolagus cuniculus (Rabbit)
59	HH	eS7	polymer	194	22168.9	1	UniProt (G1SVB0) Pfam (PF01251) In PDB	Oryctolagus cuniculus (Rabbit)
60	II	eS8	polymer	208	24263.4	1	UniProt (G1TJW1) Pfam (PF01201) In PDB	Oryctolagus cuniculus (Rabbit)
61	JJ	uS4	polymer	194	22641.6	1	UniProt (B7NZS8) Pfam (PF00163) Pfam (PF01479) In PDB	Oryctolagus cuniculus (Rabbit)
62	KK	eS10	polymer	165	18933.8	1	UniProt (G1TPV3) Pfam (PF03501) In PDB	Oryctolagus cuniculus (Rabbit)
63	LL	uS17	polymer	158	18468.8	1	UniProt (G1TRM4) Pfam (PF16205) Pfam (PF00366) In PDB	Oryctolagus cuniculus (Rabbit)
64	MM	eS12	polymer	132	14539.0	1	UniProt (G1SFR8) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
65	NN	uS15	polymer	151	17259.4	1	UniProt (G1SP51) Pfam (PF08069) Pfam (PF00312) In PDB	Oryctolagus cuniculus (Rabbit)
66	OO	uS11	polymer	168	18134.0	1	UniProt (G1T1F0) Pfam (PF00411) In PDB	Oryctolagus cuniculus (Rabbit)
67	PP	uS19	polymer	145	17049.2	1	UniProt (G1U0Q2) Pfam (PF00203) In PDB	Oryctolagus cuniculus (Rabbit)
68	QQ	uS9	polymer	146	16477.4	1	UniProt (G1SGX4) Pfam (PF00380) In PDB	Oryctolagus cuniculus (Rabbit)
69	RR	eS17	polymer	135	15552.1	1	UniProt (G1TU13) Pfam (PF00833) In PDB	Oryctolagus cuniculus (Rabbit)
70	SS	uS13	polymer	152	17759.8	1	UniProt (G1TPG3) Pfam (PF00416) In PDB	Oryctolagus cuniculus (Rabbit)
71	TT	eS19	polymer	145	16106.6	1	UniProt (G1TN62) Pfam (PF01090) In PDB	Oryctolagus cuniculus (Rabbit)
72	UU	uS10	polymer	119	13398.8	1	UniProt (G1SIZ2) Pfam (PF00338) In PDB	Oryctolagus cuniculus (Rabbit)
73	VV	eS21	polymer	83	9124.4	1		Oryctolagus cuniculus (Rabbit)
74	WW	uS8	polymer	130	14865.6	1	UniProt (G1TG89) Pfam (PF00410) In PDB	Oryctolagus cuniculus (Rabbit)
75	XX	uS12	polymer	143	15844.7	1		Oryctolagus cuniculus (Rabbit)
76	YY	eS24	polymer	130	15107.9	1		Oryctolagus cuniculus (Rabbit)
77	ZZ	eS25	polymer	125	13776.2	1	UniProt (G1TDB3) Pfam (PF03297) In PDB	Oryctolagus cuniculus (Rabbit)
78	aa	eS26	polymer	115	13047.5	1		Oryctolagus cuniculus (Rabbit)
79	bb	eS27	polymer	84	9480.2	1	UniProt (G1TZ76) Pfam (PF01667) In PDB	Oryctolagus cuniculus (Rabbit)
80	cc	eS28	polymer	69	7855.1	1	UniProt (G1TIB4) Pfam (PF01200) In PDB	Oryctolagus cuniculus (Rabbit)
81	dd	uS14	polymer	56	6690.8	1	UniProt (G1U7M4) Pfam (PF00253) In PDB	Oryctolagus cuniculus (Rabbit)
82	ee	eS30	polymer	133	14498.9	1	UniProt (G1T8A2) Pfam (PF00240) Pfam (PF04758) In PDB	Oryctolagus cuniculus (Rabbit)
83	ff	eS31	polymer	156	18004.0	1	UniProt (G1SK22) Pfam (PF00240) Pfam (PF01599) In PDB	Oryctolagus cuniculus (Rabbit)
84	gg	RACK1	polymer	317	35115.7	1	UniProt (G1SJB4) Pfam (PF00400) In PDB	Oryctolagus cuniculus (Rabbit)
85	hh	mRNA (UGA stop codon)	polymer	15	4753.9	1		Oryctolagus cuniculus
86	ii	eRF1(AAQ)	polymer	459	51667.6	1	UniProt (P62495) Pfam (PF03463) Pfam (PF03464) Pfam (PF03465) In PDB	Homo sapiens (Human)	eRF1,Protein Cl1,TB3-1
87	jj	ABCE1	polymer	599	67405.2	1	UniProt (G1SG72) Pfam (PF04068) Pfam (PF00037) Pfam (PF00005) In PDB	Oryctolagus cuniculus (Rabbit)
88	5, 9, hh, B, I...	MAGNESIUM ION	non-polymer		24.3	300	Chemie (MG)
89	aa, dd, ff, g, j...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
90	jj	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

B: 1 - 403 (UniProt: G1TL06)

PDB	External Database	Details
Met 1	Gln 1	initiating methionine

C: 1 - 378 (UniProt: G1SVW5)
C: 379 - 425 (PDB: 5LZV)
D: 1 - 297 (UniProt: G1SYJ6)

PDB	External Database	Details
Met 1	Lys 1	initiating methionine

F: 1 - 247 (UniProt: G1TUB1)

PDB	External Database	Details
Arg 61	Gly 61	conflict
Arg 93	Gly 93	conflict
Met 131	Val 131	conflict
Ile 153	Val 153	conflict

G: 1 - 77 (PDB: 5LZV)
G: 78 - 319 (UniProt: G1STW0)

PDB	External Database	Details
Gly 244	Cys 167	conflict

a: 1 - 148 (UniProt: G1SNY0)

PDB	External Database	Details
Met 1	Gln 1	conflict

b: 1 - 226 (UniProt: G1SGR6)
b: 227 - 245 (PDB: 5LZV)
g: 1 - 116 (UniProt: G1U945)

PDB	External Database	Details
Lys 117	-	insertion

II: 1 - 208 (UniProt: G1TJW1)

PDB	External Database	Details
Arg 47	Gly 47	conflict

OO: -16 - -5 (PDB: 5LZV)
OO: -4 - 151 (UniProt: G1T1F0)
TT: 1 - 145 (UniProt: G1TN62)

PDB	External Database	Details
Gly 119	Trp 119	conflict

ii: 1 - 437 (UniProt: P62495)

PDB	External Database	Details
Met -21	-	initiating methionine
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Met -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 183	Gly 183	conflict
Ala 184	Gly 184	conflict

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	87
Polymers	Total formula weight	3535563.1
Non-Polymers*	Number of molecules	310
Non-Polymers*	Total formula weight	8518.1
All*	Total formula weight	3544081.2

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)