5I95
Crystal Structure of Human Mitochondrial Isocitrate Dehydrogenase R140Q Mutant Homodimer bound to NADPH and alpha-Ketoglutaric acid
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Isocitrate dehydrogenase [NADP], mitochondrial | polymer | 424 | 48130.9 | 1 | UniProt (P48735) Pfam (PF00180) In PDB | Homo sapiens (Human) | IDH,ICD-M,IDP,NADP(+)-specific ICDH,Oxalosuccinate decarboxylase |
2 | A | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
3 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | A | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
5 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
6 | A | 2-OXOGLUTARIC ACID | non-polymer | 146.1 | 1 | Chemie (AKG) | |||
7 | water | water | 18.0 | 589 | Chemie (HOH) |
Sequence modifications
A: 40 - 452 (UniProt: P48735)
PDB | External Database | Details |
---|---|---|
Met 39 | - | initiating methionine |
Gln 140 | Arg 140 | engineered mutation |
Leu 453 | - | expression tag |
Glu 454 | - | expression tag |
His 455 | - | expression tag |
His 456 | - | expression tag |
His 457 | - | expression tag |
His 458 | - | expression tag |
His 459 | - | expression tag |
His 460 | - | expression tag |
His 461 | - | expression tag |
His 462 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 48130.9 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 1359.0 | |
All* | Total formula weight | 49489.9 |