5H02
Crystal structure of Methanohalophilus portucalensis glycine sarcosine N-methyltransferase tetramutant (H21G, E23T, E24N, L28S)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Glycine sarcosine N-methyltransferase | polymer | 283 | 32518.9 | 1 | UniProt (F6KV61) Pfam (PF13649) In PDB | Methanohalophilus portucalensis FDF-1 | |
2 | A | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
3 | A | TRIMETHYL GLYCINE | non-polymer | 118.2 | 2 | Chemie (BET) | |||
4 | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A: 1 - 263 (UniProt: F6KV61)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Gly 21 | His 21 | engineered mutation |
Thr 23 | Glu 23 | engineered mutation |
Asn 24 | Glu 24 | engineered mutation |
Ser 28 | Leu 28 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 32518.9 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 620.7 | |
All* | Total formula weight | 33139.6 |