4IK9

High resolution structure of GCaMP3 dimer form 2 at pH 7.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RCaMP, Green fluorescent protein	polymer	450	50544.4	1	UniProt (K4DIE3) UniProt (P42212) Pfam (PF01353) Pfam (PF13499) In PDB	Entacmaea quadricolor (Bubble-tip anemone)
2	A	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	465	Chemie (HOH)

Sequence modifications

A: 11 - 58 (UniProt: K4DIE3)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Gly 10	-	expression tag

A: 61 - 150 (UniProt: P42212)

PDB	External Database	Details
Lys 65	Met 153	engineered mutation
Ala 75	Val 163	engineered mutation
Gly 87	Ser 175	engineered mutation
Tyr 92	Asp 180	engineered mutation
Val 115	Thr 203	engineered mutation
Lys 118	Ala 206	engineered mutation
Leu 143	His 231	engineered mutation

A: 159 - 301 (UniProt: P42212)

PDB	External Database	Details
Gly 151	-	linker
Gly 152	-	linker
Thr 153	-	linker
Gly 154	-	linker
Gly 155	-	linker
Ser 156	-	linker
Met 157	-	linker
Val 158	-	linker
Leu 221	Phe 64	engineered mutation
Cro 222	Ser 65	chromophore
Cro 222	Tyr 66	chromophore
Cro 222	Gly 67	chromophore
Ile 250	Val 93	engineered mutation

A: 302 - 450 (UniProt: K4DIE3)

PDB	External Database	Details
Leu 59	-	linker
Glu 60	-	linker
Thr 372	Ile 354	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50544.4
Non-Polymers*	Number of molecules	5
	Total formula weight	266.4
All*	Total formula weight	50810.8

*Water molecules are not included.