4D5N

Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1	polymer	436	49040.7	1	UniProt (P62495) Pfam (PF03463) Pfam (PF03464) Pfam (PF03465) In PDB	HOMO SAPIENS (HUMAN)	EUKARYOTIC RELEASE FACTOR 1, ERF1, PROTEIN CL1, TB3-1, EUKARYORIC RELEASE FACTOR 1
2	X	CRICKET PARALYSIS VIRUS IRES RNA	polymer	201	64363.9	1	GenBank (8895506)	CRICKET PARALYSIS VIRUS

Sequence modifications

A: 5 - 437 (UniProt: P62495)

PDB	External Database	Details
Leu 438	-	expression tag
Glu 439	-	expression tag
His 440	-	expression tag

X: 6030 - 6216 (GenBank: 8895506)

PDB	External Database	Details
A 6178	U 6178	conflict

X: 6217 - 6230 (PDB: 4D5N)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	113404.6
All*	Total formula weight	113404.6

*Water molecules are not included.