3P5T

CFIm25-CFIm68 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Cleavage and polyadenylation specificity factor subunit 5	polymer	202	23614.1	6	UniProt (O43809) Pfam (PF13869) In PDB	Homo sapiens (human)	Cleavage factor Im 25, Cleavage and polyadenylation specificity factor 25 kDa subunit, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit
2	L, M, N, O, P...	Cleavage and polyadenylation specificity factor subunit 6	polymer	90	10149.4	6	UniProt (Q16630) Pfam (PF00076) In PDB	Homo sapiens (human)	Cleavage factor Im 68, Cleavage and polyadenylation specificity factor 68 kDa subunit, CF Im68, CPSF 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7
3		water	water		18.0	248	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 34 - 227 (UniProt: O43809)

PDB	External Database	Details
Leu 228	-	expression tag
Glu 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag

L, M, N, O, P, Q: 80 - 161 (UniProt: Q16630)

PDB	External Database	Details
Ser 159	Cys 159	engineered mutation
Leu 162	-	expression tag
Glu 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	202581.2
All*	Total formula weight	202581.2

*Water molecules are not included.