3P56

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Ribonuclease H2 subunit A	polymer	299	33343.8	2	UniProt (O75792) Pfam (PF01351) In PDB	Homo sapiens (human)	RNase H2 subunit A, Aicardi-Goutieres syndrome 4 protein, AGS4, RNase H(35), Ribonuclease HI large subunit, RNase HI large subunit, Ribonuclease HI subunit A
2	B, E	Ribonuclease H2 subunit B	polymer	237	26699.7	2	UniProt (Q5TBB1) Pfam (PF17745) Pfam (PF09468) In PDB	Homo sapiens (human)	RNase H2 subunit B, Aicardi-Goutieres syndrome 2 protein, AGS2, Deleted in lymphocytic leukemia 8, Ribonuclease HI subunit B
3	C, F	Ribonuclease H2 subunit C	polymer	164	17862.1	2	UniProt (Q8TDP1) Pfam (PF08615) In PDB	Homo sapiens (human)	RNase H2 subunit C, Aicardi-Goutieres syndrome 3 protein, AGS3, RNase H1 small subunit, Ribonuclease HI subunit C

Sequence modifications

A, D: 1 - 299 (UniProt: O75792)

PDB	External Database	Details
Ala 34	Asp 34	engineered mutation
Ala 169	Asp 169	engineered mutation

B, E: 2 - 226 (UniProt: Q5TBB1)

PDB	External Database	Details
Gly -10	-	expression tag
Pro -9	-	expression tag
Leu -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Pro -5	-	expression tag
Glu -4	-	expression tag
Phe -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Leu 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	155811.3
All*	Total formula weight	155811.3

*Water molecules are not included.