2IW8

STRUCTURE OF HUMAN THR160-PHOSPHO CDK2-CYCLIN A F82H-L83V-H84D MUTANT WITH AN O6-CYCLOHEXYLMETHYLGUANINE INHIBITOR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	CELL DIVISION PROTEIN KINASE 2	polymer	302	34308.7	2	UniProt (P24941) Pfam (PF00069) In PDB	HOMO SAPIENS (HUMAN)	CYCLIN DEPENDENT KINASE 2, P33 PROTEIN KINASE
2	B, D	CYCLIN-A2	polymer	260	29884.6	2	UniProt (P20248) Pfam (PF00134) Pfam (PF02984) In PDB	HOMO SAPIENS (HUMAN)	HUMAN CYCLIN A, CYCLIN-A
3	A, C	O6-CYCLOHEXYLMETHOXY-2-(4'-SULPHAMOYLANILINO) PURINE	non-polymer		402.5	2	Chemie (4SP)
4	B, D	MONOTHIOGLYCEROL	non-polymer		108.2	2	Chemie (SGM)
5		water	water		18.0	309	Chemie (HOH)

Sequence modifications

A, C: 1 - 298 (UniProt: P24941)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 82	Phe 82	engineered mutation
Val 83	Leu 83	engineered mutation
Asp 84	His 84	engineered mutation

B, D: 174 - 432 (UniProt: P20248)

PDB	External Database	Details
Met 173	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128386.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1021.3
All*	Total formula weight	129407.8

*Water molecules are not included.