2FYD
catalytic domain of bovine beta 1, 4-galactosyltransferase in complex with alpha-lactalbumin, glucose, Mn, and UDP-N-acetylgalactosamine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C | Alpha-lactalbumin | polymer | 123 | 14015.8 | 2 | UniProt (P29752) Pfam (PF00062) In PDB | Mus musculus (house mouse) | Lactose synthase B protein |
| 2 | B, D | beta-1,4-galactosyltransferase | polymer | 286 | 32772.5 | 2 | GenBank (21450879) Pfam (PF13733) Pfam (PF02709) UniProt (by SIFTS) (P08037) In PDB | Bos taurus (cattle) | |
| 3 | A, C | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | A, C | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
| 5 | A, C | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 6 | B, D | beta-D-glucopyranose | non-polymer | 180.2 | 2 | Chemie (BGC) | |||
| 7 | B, D | 2-acetamido-2-deoxy-beta-D-galactopyranose | non-polymer | 221.2 | 2 | Chemie (NGA) | |||
| 8 | B, D | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 9 | B, D | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 2 | Chemie (UDP) | |||
| 10 | water | water | 18.0 | 509 | Chemie (HOH) |
Sequence modifications
B, D: 130 - 402 (GenBank: 21450879)
| PDB | External Database | Details |
|---|---|---|
| Ala 117 | - | cloning artifact |
| Ser 118 | - | cloning artifact |
| Met 119 | - | cloning artifact |
| Thr 120 | - | cloning artifact |
| Gly 121 | - | cloning artifact |
| Gly 122 | - | cloning artifact |
| Gln 123 | - | cloning artifact |
| Gln 124 | - | cloning artifact |
| Met 125 | - | cloning artifact |
| Gly 126 | - | cloning artifact |
| Arg 127 | - | cloning artifact |
| Gly 128 | - | cloning artifact |
| Ser 129 | - | cloning artifact |
| Cys 312 | Trp 239 | engineered mutation |
| Thr 342 | Cys 269 | engineered mutation |
| Cys 401 | Pro 328 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 93576.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 2580.0 | |
| All* | Total formula weight | 96156.8 |
