1O0R

Crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase complex with UDP-galactose

Replaces:  1KYB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	beta-1,4-galactosyltransferase	polymer	286	32849.6	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709) In PDB	Bos taurus (cattle)
2	A, B	GALACTOSE-URIDINE-5'-DIPHOSPHATE	non-polymer		566.3	2	Chemie (GDU)
3	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	A, B	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
7		water	water		18.0	439	Chemie (HOH)

Sequence modifications

A, B: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	SEE REMARK 999
Ser 118	-	SEE REMARK 999
Met 119	-	SEE REMARK 999
Thr 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Gly 122	-	SEE REMARK 999
Gln 123	-	SEE REMARK 999
Gln 124	-	SEE REMARK 999
Met 125	-	SEE REMARK 999
Gly 126	-	SEE REMARK 999
Arg 127	-	SEE REMARK 999
Gly 128	-	SEE REMARK 999
Ser 129	-	SEE REMARK 999
Thr 342	Cys 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65699.2
Non-Polymers*	Number of molecules	11
	Total formula weight	1998.2
All*	Total formula weight	67697.3

*Water molecules are not included.