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1CFR
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BU of 1cfr by Molmil
CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.
Descriptor: RESTRICTION ENDONUCLEASE
Authors:Bozic, D, Grazulis, S, Siksnys, V, Huber, R.
Deposit date:1996-02-02
Release date:1997-01-27
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution.
J.Mol.Biol., 255, 1996
1KNV
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BU of 1knv by Molmil
Bse634I restriction endonuclease
Descriptor: ACETATE ION, Bse634I restriction endonuclease, CHLORIDE ION
Authors:Grazulis, S, Deibert, M, Rimseliene, R, Skirgaila, R, Sasnauskas, G, Lagunavicius, A, Repin, V, Urbanke, C, Huber, R, Siksnys, V.
Deposit date:2001-12-19
Release date:2002-02-27
Last modified:2024-03-13
Method:X-RAY DIFFRACTION (2.17 Å)
Cite:Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.
Nucleic Acids Res., 30, 2002
3DPG
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BU of 3dpg by Molmil
SgrAI with noncognate DNA bound
Descriptor: CALCIUM ION, DNA (5'-D(*DAP*DAP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DT)-3'), SgraIR restriction enzyme
Authors:Dunten, P.W, Horton, N.C, Little, E.J.
Deposit date:2008-07-08
Release date:2008-08-19
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (1.91 Å)
Cite:The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
3DVO
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BU of 3dvo by Molmil
SgrAI with cognate DNA and calcium bound
Descriptor: CALCIUM ION, DNA (5'-D(*DGP*DAP*DGP*DTP*DCP*DCP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DTP*DC)-3'), SgraIR restriction enzyme
Authors:Dunten, P.W, Horton, N.C, Little, E.J.
Deposit date:2008-07-18
Release date:2008-08-19
Last modified:2023-08-30
Method:X-RAY DIFFRACTION (1.892 Å)
Cite:The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
3DW9
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BU of 3dw9 by Molmil
SgrAI with cognate DNA and manganese bound
Descriptor: DNA (5'-D(*DGP*DAP*DGP*DTP*DCP*DCP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DTP*DC)-3'), MANGANESE (II) ION, SgraIR restriction enzyme
Authors:Dunten, P.W, Horton, N.C, Little, E.J.
Deposit date:2008-07-21
Release date:2008-08-19
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:The structure of SgrAI bound to DNA; recognition of an 8 base pair target.
Nucleic Acids Res., 36, 2008
3MQ6
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BU of 3mq6 by Molmil
Domain swapped SgrAI with DNA and calcium bound
Descriptor: CALCIUM ION, DNA (5'-D(*AP*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), SgraIR restriction enzyme
Authors:Dunten, P.W, Horton, N.C, Little, E.J.
Deposit date:2010-04-27
Release date:2010-11-10
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2 Å)
Cite:Domain swapping in allosteric modulation of DNA specificity.
Plos Biol., 8, 2010
3MQY
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BU of 3mqy by Molmil
SgrAI with cleaved DNA and Magnesium bound
Descriptor: DNA (5'-D(*GP*AP*GP*TP*CP*CP*A)-3'), DNA (5'-D(P*CP*CP*GP*GP*TP*GP*GP*AP*CP*TP*C)-3'), MAGNESIUM ION, ...
Authors:Dunten, P.W, Horton, N.C, Little, E.J.
Deposit date:2010-04-28
Release date:2010-11-24
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (2 Å)
Cite:New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
3N7B
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BU of 3n7b by Molmil
SgrAI bound to secondary site DNA and Ca(II)
Descriptor: CALCIUM ION, DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), ...
Authors:Horton, N.C, Little, E.J, Dunten, P.W.
Deposit date:2010-05-26
Release date:2010-11-24
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.65 Å)
Cite:New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
3N78
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BU of 3n78 by Molmil
SgrAI bound to Secondary Site DNA and Mg(II)
Descriptor: DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3'), DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3'), MAGNESIUM ION, ...
Authors:Horton, N.C, Little, E.J, Dunten, P.W.
Deposit date:2010-05-26
Release date:2010-11-24
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.95 Å)
Cite:New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.
Acta Crystallogr.,Sect.D, 67, 2011
3V1Z
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BU of 3v1z by Molmil
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Descriptor: DNA (5'-D(*TP*CP*GP*CP*GP*CP*CP*GP*GP*CP*GP*CP*G)-3'), Endonuclease Bse634IR
Authors:Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G.
Deposit date:2011-12-11
Release date:2012-04-25
Last modified:2023-09-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
3V21
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BU of 3v21 by Molmil
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Descriptor: DNA (5'-D(*TP*TP*CP*GP*AP*CP*CP*GP*GP*TP*CP*GP*A)-3'), Endonuclease Bse634IR
Authors:Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G.
Deposit date:2011-12-11
Release date:2012-04-25
Last modified:2023-09-13
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
3V20
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BU of 3v20 by Molmil
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Descriptor: 2-(2-(2-(2-(2-(2-ETHOXYETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHOXY)ETHANOL, CALCIUM ION, CHLORIDE ION, ...
Authors:Manakova, E.N, Grazulis, S, Golovenko, D, Tamulaitiene, G.
Deposit date:2011-12-11
Release date:2012-04-25
Last modified:2023-09-13
Method:X-RAY DIFFRACTION (2.35 Å)
Cite:Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease.
Nucleic Acids Res., 40, 2012
4C3G
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BU of 4c3g by Molmil
cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI
Descriptor: 5'-D(*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*CP *CP*AP*CP*GP*CP*AP*TP*CP)-3', 5'-D(*GP*AP*TP*GP*CP*GP*TP*GP*GP*GP*TP*CP*TP*TP *CP*AP*CP*AP)-3', SGRAIR RESTRICTION ENZYME
Authors:Lyumkis, D, Talley, H, Stewart, A, Shah, S, Park, C.K, Tama, F, Potter, C.S, Carragher, B, Horton, N.C.
Deposit date:2013-08-23
Release date:2013-09-11
Last modified:2017-08-23
Method:ELECTRON MICROSCOPY (8.6 Å)
Cite:Allosteric Regulation of DNA Cleavage and Sequence-Specificity Through Run-on Oligomerization.
Structure, 21, 2013
6OBJ
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BU of 6obj by Molmil
Structure of a DNA-bound dimer extracted from filamentous SgrAI endonuclease in its activated form
Descriptor: DNA (26-MER), MAGNESIUM ION, SgraIR restriction enzyme
Authors:Polley, S, Lyumkis, D, Horton, N.C.
Deposit date:2019-03-20
Release date:2020-02-26
Last modified:2024-03-20
Method:ELECTRON MICROSCOPY (3.5 Å)
Cite:Mechanism of Filamentation-Induced Allosteric Activation of the SgrAI Endonuclease.
Structure, 27, 2019
7S8D
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BU of 7s8d by Molmil
Structure of DNA-free SgrAI
Descriptor: CALCIUM ION, SgraIR restriction enzyme
Authors:Horton, N.C.
Deposit date:2021-09-17
Release date:2022-08-24
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (2.02 Å)
Cite:Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity.
J.Biol.Chem., 298, 2022
7SS5
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BU of 7ss5 by Molmil
Activated SgrAI endonuclease DNA-bound dimer with Ca2+ and intact primary site DNA
Descriptor: CALCIUM ION, DNA (5'-D(P*GP*GP*TP*CP*TP*TP*CP*AP*CP*AP*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*C)-3'), SgraIR restriction enzyme
Authors:Shan, Z, Lyumkis, D, Horton, N.C.
Deposit date:2021-11-09
Release date:2021-12-01
Last modified:2022-06-15
Method:ELECTRON MICROSCOPY (2.7 Å)
Cite:Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity.
J.Biol.Chem., 298, 2022

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