1R7M
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THE HOMING ENDONUCLEASE I-SCEI BOUND TO ITS DNA RECOGNITION REGION
Descriptor:5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*AP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*TP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', Intron-encoded endonuclease I-SceI, ...
Authors:Moure, C.M., Gimble, F.S., Quiocho, F.A.
Deposit date:2003-10-21
Release date:2004-10-26
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity
J.Mol.Biol., 334, 2003
3C0W
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I-SCEI IN COMPLEX WITH A BOTTOM NICKED DNA SUBSTRATE
Descriptor:DNA (5'-D(*DAP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DAP*DAP*DCP*DAP*DGP*DGP*DGP*DTP*DAP*DAP*DTP*DAP*DC)-3'), DNA (5'-D(*DGP*DTP*DAP*DTP*DTP*DAP*DCP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DT)-3'), DNA (5'-D(P*DCP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DT)-3'), ...
Authors:Moure, C.M., Gimble, F.S., Quiocho, F.A.
Deposit date:2008-01-21
Release date:2008-05-06
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Nucleic Acids Res., 36, 2008
3C0X
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I-SCEI IN COMPLEX WITH A TOP NICKED DNA SUBSTRATE
Descriptor:DNA (5'-D(*DC*DAP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DAP*DA)-3'), DNA (5'-D(P*DCP*DAP*DGP*DGP*DGP*DTP*DAP*DAP*DTP*DAP*DC)-3'), DNA (5'-D(*DG*DGP*DTP*DAP*DTP*DTP*DAP*DCP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DTP*DCP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DT)-3'), ...
Authors:Moure, C.M., Gimble, F.S., Quiocho, F.A.
Deposit date:2008-01-21
Release date:2008-05-06
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Nucleic Acids Res., 36, 2008
3OOL
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I-SCEI COMPLEXED WITH C/G+4 DNA SUBSTRATE
Descriptor:Intron encoded endonuclease I-SceI, 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', ...
Authors:Joshi, R., Chen, J.-H., Golden, B.L., Gimble, F.S.
Deposit date:2010-08-31
Release date:2010-11-17
Last modified:2018-01-24
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity.
J.Mol.Biol., 405, 2011
3OOR
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I-SCEI MUTANT (K86R/G100T)COMPLEXED WITH C/G+4 DNA SUBSTRATE
Descriptor:Intron encoded endonuclease I-SceI, 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', ...
Authors:Joshi, R., Chen, J.-H., Golden, B.L., Gimble, F.S.
Deposit date:2010-08-31
Release date:2010-11-17
Last modified:2018-01-24
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity.
J.Mol.Biol., 405, 2011
5A0M
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THE CRYSTAL STRUCTURE OF I-SCEI IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF MN
Descriptor:INTRON-ENCODED ENDONUCLEASE I-SCEI, 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*TP*GP*TP*TP *AP*TP)-3', ...
Authors:Prieto, J., Redondo, P., Merino, N., Villate, M., Blanco, F.J., Molina, R.
Deposit date:2015-04-21
Release date:2016-05-04
Last modified:2016-06-29
Method:X-RAY DIFFRACTION (2.9 Å)
Cite:Structure of the I-Scei Nuclease Complexed with its DsDNA Target and Three Catalytic Metal Ions.
Acta Crystallogr.,Sect.F, 72, 2016
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