1A2Q
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SUBTILISIN BPN' MUTANT 7186
Descriptor:SUBTILISIN BPN', CALCIUM ION, ACETONE
Authors:Gilliland, G.L., Whitlow, M., Howard, A.J.
Deposit date:1998-01-08
Release date:1998-04-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.
Biochemistry, 28, 1989
1AF4
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CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG IN ANHYDROUS DIOXANE
Descriptor:SUBTILISIN CARLSBERG, CALCIUM ION, 1,4-DIETHYLENE DIOXIDE
Authors:Schmitke, J.L., Stern, L.J., Klibanov, A.M.
Deposit date:1997-03-21
Release date:1997-06-16
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile.
Proc.Natl.Acad.Sci.USA, 94, 1997
1AH2
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SERINE PROTEASE PB92 FROM BACILLUS ALCALOPHILUS, NMR, 18 STRUCTURES
Descriptor:SERINE PROTEASE PB92
Authors:Boelens, R., Schipper, D., Martin, J.R., Karimi-Nejad, Y., Mulder, F., Zwan, J.V.D., Mariani, M.
Deposit date:1997-04-11
Release date:1998-04-15
Last modified:2011-07-13
Method:SOLUTION NMR
Cite:The solution structure of serine protease PB92 from Bacillus alcalophilus presents a rigid fold with a flexible substrate-binding site.
Structure, 5, 1997
1AK9
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SUBTILISIN MUTANT 8321
Descriptor:SUBTILISIN 8321, CALCIUM ION, SODIUM ION, ...
Authors:Whitlow, M., Howard, A.J., Wood, J.F.
Deposit date:1997-05-30
Release date:1997-11-12
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.
Biochemistry, 28, 1989
1AQN
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SUBTILISIN MUTANT 8324
Descriptor:SUBTILISIN 8324, CALCIUM ION, UNKNOWN ATOM OR ION, ...
Authors:Whitlow, M., Howard, A.J., Wood, J.F.
Deposit date:1997-07-31
Release date:1998-01-14
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Large increases in general stabilityfor subtilisin BPN' through incremental changes in the free energy of unfolding
To be published
1AU9
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SUBTILISIN BPN' MUTANT 8324 IN CITRATE
Descriptor:SUBTILISIN BPN', CALCIUM ION, UNKNOWN ATOM OR ION, ...
Authors:Whitlow, M., Howard, A.J., Wood, J.F.
Deposit date:1997-09-12
Release date:1997-12-31
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.
Biochemistry, 28, 1989
1AV7
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SUBTILISIN CARLSBERG L-NAPHTHYL-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX
Descriptor:SUBTILISIN CARLSBERG, TYPE VIII, SODIUM ION
Authors:Stoll, V.S., Eger, B.T., Hynes, R.C., Martichonok, V., Jones, J.B., Pai, E.F.
Deposit date:1997-09-29
Release date:1998-04-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes.
Biochemistry, 37, 1998
1AVT
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SUBTILISIN CARLSBERG D-PARA-CHLOROPHENYL-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX
Descriptor:SUBTILISIN CARLSBERG, TYPE VIII, SODIUM ION
Authors:Stoll, V.S., Eger, B.T., Hynes, R.C., Martichonok, V., Jones, J.B., Pai, E.F.
Deposit date:1997-09-19
Release date:1998-03-25
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes.
Biochemistry, 37, 1998
1BE6
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TRANS-CINNAMOYL-SUBTILISIN IN ANHYDROUS ACETONITRILE
Descriptor:SUBTILISIN CARLSBERG, CALCIUM ION, PHENYLETHYLENECARBOXYLIC ACID, ...
Authors:Schmitke, J.L., Stern, L.J., Klibanov, A.M.
Deposit date:1998-05-20
Release date:1998-10-14
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water.
Proc.Natl.Acad.Sci.USA, 95, 1998
1BE8
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TRANS-CINNAMOYL-SUBTILISIN IN WATER
Descriptor:SUBTILISIN CARLSBERG, CALCIUM ION, PHENYLETHYLENECARBOXYLIC ACID
Authors:Schmitke, J.L., Stern, L.J., Klibanov, A.M.
Deposit date:1998-05-20
Release date:1998-10-28
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water.
Proc.Natl.Acad.Sci.USA, 95, 1998
1BFK
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CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG IN 40% ACETONITRILE
Descriptor:SUBTILISIN CARLSBERG, CALCIUM ION, ACETONITRILE
Authors:Schmitke, J.L., Stern, L.J., Klibanov, A.M.
Deposit date:1998-05-21
Release date:1998-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents.
Biochem.Biophys.Res.Commun., 248, 1998
1BFU
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SUBTILISIN CARLSBERG IN 20% DIOXANE
Descriptor:SUBTILISIN CARLSBERG, CALCIUM ION, 1,4-DIETHYLENE DIOXIDE
Authors:Schmitke, J.L., Stern, L.J., Klibanov, A.M.
Deposit date:1998-05-22
Release date:1998-10-28
Last modified:2017-11-29
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents.
Biochem.Biophys.Res.Commun., 248, 1998
1BH6
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SUBTILISIN DY IN COMPLEX WITH THE SYNTHETIC INHIBITOR N-BENZYLOXYCARBONYL-ALA-PRO-PHE-CHLOROMETHYL KETONE
Descriptor:SUBTILISIN DY, CALCIUM ION, SODIUM ION, ...
Authors:Eschenburg, S., Genov, N., Wilson, K.S., Betzel, C.
Deposit date:1998-06-15
Release date:1998-11-04
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Crystal structure of subtilisin DY, a random mutant of subtilisin Carlsberg.
Eur.J.Biochem., 257, 1998
1BJR
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COMPLEX FORMED BETWEEN PROTEOLYTICALLY GENERATED LACTOFERRIN FRAGMENT AND PROTEINASE K
Descriptor:PROTEINASE K, LACTOFERRIN, CALCIUM ION
Authors:Singh, T.P., Sharma, S., Karthikeyan, S., Betzel, C., Bhatia, K.L.
Deposit date:1998-06-27
Release date:1998-11-04
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.44 Å)
Cite:Crystal structure of a complex formed between proteolytically-generated lactoferrin fragment and proteinase K.
Proteins, 33, 1998
1C3L
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SUBTILISIN-CARLSBERG COMPLEXED WITH XENON (8 BAR)
Descriptor:SUBTILISIN-CARLSBERG, CALCIUM ION, XENON, ...
Authors:Prange, T., Schiltz, M., Pernot, L., Colloc'h, N., Longhi, S.
Deposit date:1999-07-28
Release date:1999-08-04
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.16 Å)
Cite:Exploring hydrophobic sites in proteins with xenon or krypton.
Proteins, 30, 1998
1C9J
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BACILLUS LENTUS SUBTILISIN K27R/N87S/V104Y/N123S/T274A VARIANT
Descriptor:SERINE PROTEASE, SULFATE ION, CALCIUM ION
Authors:Bott, R.
Deposit date:1999-08-02
Release date:1999-10-06
Last modified:2018-04-18
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Engineered Bacillus lentus subtilisins having altered flexibility.
J.Mol.Biol., 292, 1999
1C9M
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BACILLUS LENTUS SUBTILSIN (SER 87) N76D/S103A/V104I
Descriptor:SERINE PROTEASE, SULFATE ION, CALCIUM ION
Authors:Bott, R.
Deposit date:1999-08-02
Release date:1999-10-06
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.67 Å)
Cite:Engineered Bacillus lentus subtilisins having altered flexibility.
J.Mol.Biol., 292, 1999
1C9N
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BACILLUS LENTUS SUBSTILISIN VARIANT (SER 87) K27R/V104Y/N123S/T274A
Descriptor:SERINE PROTEASE, SULFATE ION, CALCIUM ION
Authors:Bott, R.
Deposit date:1999-08-02
Release date:1999-10-06
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Engineered Bacillus lentus subtilisins having altered flexibility.
J.Mol.Biol., 292, 1999
1CNM
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ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOSURE TO ANHYDROUS ORGANIC SOLVENT AT 70 DEGREES CELSIUS
Descriptor:PROTEIN (PROTEINASE K), CALCIUM ION, ACETONITRILE
Authors:Gupta, M.N., Tyagi, R., Sharma, S., Karthikeyan, S., Singh, T.P.
Deposit date:1999-05-20
Release date:1999-05-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Enhancement of catalytic efficiency of enzymes through exposure to anhydrous organic solvent at 70 degrees C. Three-dimensional structure of a treated serine proteinase at 2.2 A resolution.
Proteins, 39, 2000
1DBI
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CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE PROTEASE
Descriptor:AK.1 SERINE PROTEASE, CALCIUM ION, SODIUM ION
Authors:Smith, C.A., Toogood, H.S., Baker, H.M., Daniel, R.M., Baker, E.N.
Deposit date:1999-11-02
Release date:1999-11-18
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 A resolution.
J.Mol.Biol., 294, 1999
1DUI
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SUBTILISIN BPN' FROM BACILLUS AMYLOLIQUEFACIENS, CRYSTAL GROWTH MUTANT
Descriptor:PROTEIN (SUBTILISIN BPN'), SODIUM ION, DIISOPROPYL PHOSPHONATE
Authors:Pan, Q., Gallagher, D.T.
Deposit date:2000-01-17
Release date:2000-01-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Probing Protein Interaction Chemistry Through Crystal Growth: Structure, Mutation, and Mechanism in Subtilisin s88
J.Cryst.Growth, 212, 2000
1EA7
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SPHERICASE
Descriptor:SERINE PROTEASE, CALCIUM ION
Authors:Almog, O., Gonzalez, A., Klein, D., Braun, S., Shoham, G.
Deposit date:2001-07-10
Release date:2002-07-04
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.93 Å)
Cite:The 0.93A Crystal Structure of Sphericase: A Calcium-Loaded Serine Protease from Bacillus Sphaericus
J.Mol.Biol., 332, 2003
1EGQ
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ENHANCEMENT OF ENZYME ACTIVITY THROUGH THREE-PHASE PARTITIONING: CRYSTAL STRUCTURE OF A MODIFIED SERINE PROTEINASE AT 1.5 A RESOLUTION
Descriptor:PROTEINASE K, CALCIUM ION, ACETIC ACID
Authors:Singh, R.K., Gourinath, S., Sharma, S., Ray, I., Gupta, M.N., Singh, T.P.
Deposit date:2000-02-16
Release date:2001-02-21
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:Enhancement of enzyme activity through three-phase partitioning: crystal structure of a modified serine proteinase at 1.5 A resolution.
Protein Eng., 14, 2001
1GCI
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THE 0.78 ANGSTROMS STRUCTURE OF A SERINE PROTEASE-BACILLUS LENTUS SUBTILISIN
Descriptor:SUBTILISIN, SULFATE ION, CALCIUM ION, ...
Authors:Bott, R., Kuhn, P.
Deposit date:1998-09-02
Release date:1998-10-21
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.78 Å)
Cite:The 0.78 A structure of a serine protease: Bacillus lentus subtilisin.
Biochemistry, 37, 1998
1GNS
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SUBTILISIN BPN'
Descriptor:SUBTILISIN BPN', ACETONE
Authors:Almog, O., Gallagher, D.T., Ladner, J.E., Strausberg, S., Alexander, P.
Deposit date:2001-10-06
Release date:2002-06-27
Last modified:2015-04-15
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Structural Basis of Thermostability. Analysis of Stabilizing Mutations in Subtilisin Bpn'.
J.Biol.Chem., 277, 2002
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