7SW7
| MicroED structure of proteinase K from a 530 nm thick lamella measured at 200 kV | Descriptor: | Proteinase K | Authors: | Martynowycz, M.W, Clabbers, M.T.B, Unge, J, Hattne, J, Gonen, T. | Deposit date: | 2021-11-19 | Release date: | 2022-09-07 | Last modified: | 2023-10-18 | Method: | ELECTRON CRYSTALLOGRAPHY (2.3 Å) | Cite: | Benchmarking the ideal sample thickness in cryo-EM. Proc.Natl.Acad.Sci.USA, 118, 2021
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7SW0
| MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV | Descriptor: | Proteinase K | Authors: | Martynowycz, M.W, Clabbers, M.T.B, Unge, J, Hattne, J, Gonen, T. | Deposit date: | 2021-11-19 | Release date: | 2022-09-07 | Last modified: | 2023-10-18 | Method: | ELECTRON CRYSTALLOGRAPHY (2.7 Å) | Cite: | Benchmarking the ideal sample thickness in cryo-EM. Proc.Natl.Acad.Sci.USA, 118, 2021
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7SW5
| MicroED structure of proteinase K from a 460 nm thick lamella measured at 200 kV | Descriptor: | Proteinase K | Authors: | Martynowycz, M.W, Clabbers, M.T.B, Unge, J, Hattne, J, Gonen, T. | Deposit date: | 2021-11-19 | Release date: | 2022-09-07 | Last modified: | 2023-10-18 | Method: | ELECTRON CRYSTALLOGRAPHY (1.95 Å) | Cite: | Benchmarking the ideal sample thickness in cryo-EM. Proc.Natl.Acad.Sci.USA, 118, 2021
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7SWC
| MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV | Descriptor: | Proteinase K | Authors: | Martynowycz, M.W, Clabbers, M.T.B, Unge, J, Hattne, J, Gonen, T. | Deposit date: | 2021-11-19 | Release date: | 2022-09-07 | Last modified: | 2023-10-18 | Method: | ELECTRON CRYSTALLOGRAPHY (2.9 Å) | Cite: | Benchmarking the ideal sample thickness in cryo-EM. Proc.Natl.Acad.Sci.USA, 118, 2021
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6Y5S
| Crystal structure of savinase at cryogenic conditions | Descriptor: | CALCIUM ION, SODIUM ION, Subtilisin Savinase | Authors: | Wu, S, Moroz, O, Turkenburg, J, Nielsen, J.E, Wilson, K.S, Teilum, K. | Deposit date: | 2020-02-25 | Release date: | 2020-06-17 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (0.95 Å) | Cite: | Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis. Peerj, 8, 2020
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6Y5T
| Crystal structure of savinase at room temperature | Descriptor: | CALCIUM ION, SODIUM ION, Subtilisin Savinase | Authors: | Wu, S, Moroz, O, Turkenburg, J, Nielsen, J.E, Wilson, K.S, Teilum, K. | Deposit date: | 2020-02-25 | Release date: | 2020-06-17 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (1.1 Å) | Cite: | Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis. Peerj, 8, 2020
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6ZET
| Crystal structure of proteinase K nanocrystals by electron diffraction with a 20 micrometre C2 condenser aperture | Descriptor: | CALCIUM ION, Proteinase K | Authors: | Evans, G, Zhang, P, Beale, E.V, Waterman, D.G. | Deposit date: | 2020-06-16 | Release date: | 2020-10-14 | Last modified: | 2024-02-14 | Method: | ELECTRON CRYSTALLOGRAPHY (2.701 Å) | Cite: | A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction. Front Mol Biosci, 7, 2020
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6ZEV
| Crystal structure of proteinase K lamellae by electron diffraction with a 20 micrometre C2 condenser aperture | Descriptor: | CALCIUM ION, Proteinase K | Authors: | Evans, G, Zhang, P, Beale, E.V, Waterman, D.G. | Deposit date: | 2020-06-16 | Release date: | 2020-10-14 | Last modified: | 2024-02-14 | Method: | ELECTRON CRYSTALLOGRAPHY (2.4 Å) | Cite: | A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction. Front Mol Biosci, 7, 2020
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6ZEU
| Crystal structure of proteinase K lamella by electron diffraction with a 50 micrometre C2 condenser aperture | Descriptor: | CALCIUM ION, Proteinase K | Authors: | Evans, G, Zhang, P, Beale, E.V, Waterman, D.G. | Deposit date: | 2020-06-16 | Release date: | 2020-10-14 | Last modified: | 2024-02-14 | Method: | ELECTRON CRYSTALLOGRAPHY (2.004 Å) | Cite: | A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction. Front Mol Biosci, 7, 2020
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3T41
| 1.95 Angstrom Resolution Crystal Structure of Epidermin Leader Peptide Processing Serine Protease (EpiP) S393A Mutant from Staphylococcus aureus | Descriptor: | CALCIUM ION, CHLORIDE ION, Epidermin leader peptide processing serine protease EpiP | Authors: | Minasov, G, Kuhn, M, Ruan, J, Halavaty, A, Shuvalova, L, Dubrovska, I, Winsor, J, Bagnoli, F, Falugi, F, Bottomley, M, Grandi, G, Anderson, W.F, Center for Structural Genomics of Infectious Diseases (CSGID) | Deposit date: | 2011-07-25 | Release date: | 2011-08-17 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (1.95 Å) | Cite: | 1.95 Angstrom Resolution Crystal Structure of Epidermin Leader Peptide Processing Serine Protease (EpiP) S393A Mutant from Staphylococcus aureus. TO BE PUBLISHED
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3TI9
| Crystal structure of the basic protease BprB from the ovine footrot pathogen, Dichelobacter nodosus | Descriptor: | CALCIUM ION, CHLORIDE ION, GLYCEROL, ... | Authors: | Wong, W, Whisstock, J.C, Porter, C.J. | Deposit date: | 2011-08-20 | Release date: | 2011-10-19 | Last modified: | 2018-01-24 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | S1 Pocket of a Bacterially Derived Subtilisin-like Protease Underpins Effective Tissue Destruction. J.Biol.Chem., 286, 2011
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7A68
| proteinase K crystallized from 0.5 M NaNO3 | Descriptor: | Proteinase K, SODIUM ION | Authors: | Ilina, K.B, Kulikov, A.G, Timofeev, V.I, Marchenkova, M.A, Pisarevsky, Y.V, Kovalchuk, M.V. | Deposit date: | 2020-08-25 | Release date: | 2020-10-21 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.55 Å) | Cite: | proteinase K crystallized from 0.5 M NaNO3 To Be Published
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3TI7
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7A9F
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7A9K
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7A9M
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3UNX
| Bond length analysis of asp, glu and his residues in subtilisin Carlsberg at 1.26A resolution | Descriptor: | CALCIUM ION, GLYCEROL, SODIUM ION, ... | Authors: | Fisher, S.J, Helliwell, J.R, Blakeley, M.P, Cianci, M, McSweeny, S. | Deposit date: | 2011-11-16 | Release date: | 2012-06-27 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (1.26 Å) | Cite: | Protonation-state determination in proteins using high-resolution X-ray crystallography: effects of resolution and completeness. Acta Crystallogr.,Sect.D, 68, 2012
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3VHQ
| Crystal structure of the Ca6 site mutant of Pro-SA-subtilisin | Descriptor: | CALCIUM ION, Tk-subtilisin | Authors: | Uehara, R, Takeuchi, Y, Tanaka, S, Matsumura, H, Koga, Y, Takano, K, Kanaya, S. | Deposit date: | 2011-09-01 | Release date: | 2012-07-11 | Method: | X-RAY DIFFRACTION (2.15 Å) | Cite: | Requirement of Ca(2+) Ions for the Hyperthermostability of Tk-Subtilisin from Thermococcus kodakarensis Biochemistry, 51, 2012
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3VYV
| Crystal structure of subtilisin NAT at 1.36 | Descriptor: | CALCIUM ION, GLYCEROL, Subtilisin NAT | Authors: | Ushijima, H, Fuchita, N, Kajiwara, T, Motoshima, H, Ueno, G, Watanabe, K. | Deposit date: | 2012-10-03 | Release date: | 2013-10-09 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.36 Å) | Cite: | Crystal structure of subtilisin NAT at 1.36 TO BE PUBLISHED
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3VV2
| Crystal structure of complex form between S324A-subtilisin and mutant Tkpro | Descriptor: | CALCIUM ION, CHLORIDE ION, PROPEPTIDE from Tk-subtilisin, ... | Authors: | Uehara, R, Ueda, Y, You, D.J, Takano, K, Koga, Y, Kanaya, S. | Deposit date: | 2012-07-12 | Release date: | 2013-03-06 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.83 Å) | Cite: | Accelerated maturation of Tk-subtilisin by a Leu Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin Febs J., 280, 2013
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3VSB
| SUBTILISIN CARLSBERG D-NAPHTHYL-1-ACETAMIDO BORONIC ACID INHIBITOR COMPLEX | Descriptor: | SODIUM ION, SUBTILISIN CARLSBERG, TYPE VIII | Authors: | Stoll, V.S, Eger, B.T, Hynes, R.C, Martichonok, V, Jones, J.B, Pai, E.F. | Deposit date: | 1997-09-25 | Release date: | 1998-03-25 | Last modified: | 2023-08-09 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes. Biochemistry, 37, 1998
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3WIU
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3WIV
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6O44
| Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis | Descriptor: | 1,2-ETHANEDIOL, CALCIUM ION, Nattokinase, ... | Authors: | Tang, H, Shi, K, Aihara, H. | Deposit date: | 2019-02-28 | Release date: | 2019-04-10 | Last modified: | 2023-10-11 | Method: | X-RAY DIFFRACTION (1.83 Å) | Cite: | Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis. Biochem. Biophys. Res. Commun., 512, 2019
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6PKM
| MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8) | Descriptor: | Proteinase K | Authors: | Martynowycz, M.W, Zhao, W, Hattne, J, Jensen, G.J, Gonen, T. | Deposit date: | 2019-06-29 | Release date: | 2019-09-04 | Last modified: | 2019-12-18 | Method: | ELECTRON CRYSTALLOGRAPHY (2.17 Å) | Cite: | Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED. Structure, 27, 2019
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