4G0W

Human topoisomerase iibeta in complex with DNA and ametantrone

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
B	0003677	molecular_function	DNA binding
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006259	biological_process	DNA metabolic process
B	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	1
Number of Residues
Details

Chain

Residue

---

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG A 1301

Chain	Residue
A	ASP557
A	ASP559

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG A 1302

Chain	Residue
A	ASN867
A	GLY868

---

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 1301

Chain	Residue
B	ASP557
B	ASP559

---

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG B 1302

Chain	Residue
B	ASN867

---

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE AKE D 101

Chain	Residue
B	LYS505
B	LEU507
B	ASN520
B	GLU522
B	GLN778
B	MET782
D	DA12
D	DG13
D	DC14
E	DG7
E	DC8
F	DT9
B	ARG503
B	GLY504

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG D 102

Chain	Residue
D	DG18

---

site_id	AC7
Number of Residues	13
Details	BINDING SITE FOR RESIDUE AKE F 101

Chain	Residue
A	ARG503
A	LYS505
A	ILE506
A	ASN520
A	GLU522
A	GLN778
A	MET782
C	DG7
C	DC8
D	DT9
F	DA12
F	DG13
F	DC14

---

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG F 102

Chain	Residue
F	DG17

---

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKS

Chain	Residue	Details
A	LEU475-SER483

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	TYR821
B	TYR821

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	GLU477
B	GLU477

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:21778401

Chain	Residue	Details
A	ASP557
A	ASP559
B	ASP557
B	ASP559

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: Interaction with DNA

Chain	Residue	Details
A	LYS505
A	ASN508
A	ARG677
A	LYS678
A	LYS739
A	TRP947
B	LYS505
B	ASN508
B	ARG677
B	LYS678
B	LYS739
B	TRP947

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Interaction with DNA => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	TYR773
B	TYR773

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Transition state stabilizer

Chain	Residue	Details
A	ARG820
B	ARG820

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	SITE: Important for DNA bending; intercalates between base pairs of target DNA

Chain	Residue	Details
B	ILE872
A	ILE872

---

site_id	SWS_FT_FI8
Number of Residues	14
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS595
A	LYS630
A	LYS641
A	LYS671
A	LYS707
A	LYS1087
B	LYS595
B	LYS630
B	LYS641
B	LYS671
B	LYS707
B	LYS1087

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS600
A	LYS638
B	LYS600
B	LYS638

---