4C51

Crystal Structure of the Catalase-Peroxidase (KatG) R418L mutant from Mycobacterium Tuberculosis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004096	molecular_function	catalase activity
A	0004601	molecular_function	peroxidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006979	biological_process	response to oxidative stress
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0016491	molecular_function	oxidoreductase activity
A	0016677	molecular_function	oxidoreductase activity, acting on a heme group of donors, nitrogenous group as acceptor
A	0020037	molecular_function	heme binding
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0045739	biological_process	positive regulation of DNA repair
A	0046677	biological_process	response to antibiotic
A	0046872	molecular_function	metal ion binding
A	0070301	biological_process	cellular response to hydrogen peroxide
A	0070402	molecular_function	NADPH binding
A	0070404	molecular_function	NADH binding
A	0098869	biological_process	cellular oxidant detoxification
B	0004096	molecular_function	catalase activity
B	0004601	molecular_function	peroxidase activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006979	biological_process	response to oxidative stress
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0016491	molecular_function	oxidoreductase activity
B	0016677	molecular_function	oxidoreductase activity, acting on a heme group of donors, nitrogenous group as acceptor
B	0020037	molecular_function	heme binding
B	0042744	biological_process	hydrogen peroxide catabolic process
B	0045739	biological_process	positive regulation of DNA repair
B	0046677	biological_process	response to antibiotic
B	0046872	molecular_function	metal ion binding
B	0070301	biological_process	cellular response to hydrogen peroxide
B	0070402	molecular_function	NADPH binding
B	0070404	molecular_function	NADH binding
B	0098869	biological_process	cellular oxidant detoxification

Functional Information from PROSITE/UniProt

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. TAALIVGGHTF

Chain	Residue	Details
A	THR262-PHE272

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site_id	PS00436
Number of Residues	12
Details	PEROXIDASE_2 Peroxidases active site signature. GPlfIRMaWHAA

Chain	Residue	Details
A	GLY99-ALA110

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01961

Chain	Residue	Details
A	HIS108
B	HIS108

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Tryptophan radical intermediate => ECO:0000269|PubMed:18052167

Chain	Residue	Details
A	TRP321
B	TRP321

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:15231843, ECO:0000269|PubMed:16566587, ECO:0000269|PubMed:24185282, ECO:0007744|PDB:1SJ2, ECO:0007744|PDB:2CCA, ECO:0007744|PDB:2CCD, ECO:0007744|PDB:4C51

Chain	Residue	Details
A	HIS270
B	HIS270

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site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000255|HAMAP-Rule:MF_01961

Chain	Residue	Details
A	ARG104
B	ARG104

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site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255); alternate => ECO:0000255|HAMAP-Rule:MF_01961, ECO:0000269|PubMed:15231843

Chain	Residue	Details
A	TRP107
B	TRP107

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site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107); alternate => ECO:0000255|HAMAP-Rule:MF_01961, ECO:0000269|PubMed:15231843

Chain	Residue	Details
B	TYR229
B	MET255
A	TYR229
A	MET255

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