4B0T
Structure of the Pup Ligase PafA of the Prokaryotic Ubiquitin-like Modification Pathway in Complex with ADP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0010498 | biological_process | proteasomal protein catabolic process |
| A | 0016874 | molecular_function | ligase activity |
| A | 0016879 | molecular_function | ligase activity, forming carbon-nitrogen bonds |
| A | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| A | 0019941 | biological_process | modification-dependent protein catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070490 | biological_process | protein pupylation |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0010498 | biological_process | proteasomal protein catabolic process |
| B | 0016874 | molecular_function | ligase activity |
| B | 0016879 | molecular_function | ligase activity, forming carbon-nitrogen bonds |
| B | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| B | 0019941 | biological_process | modification-dependent protein catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0070490 | biological_process | protein pupylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE ADP A 1478 |
| Chain | Residue |
| A | ARG60 |
| A | ARG418 |
| A | TRP440 |
| A | PRO458 |
| A | MG1479 |
| A | HOH2053 |
| A | HOH2250 |
| A | HOH2251 |
| A | HOH2252 |
| B | ILE12 |
| B | GLY14 |
| A | THR73 |
| B | ILE15 |
| B | GLU16 |
| B | LYS444 |
| B | ASN446 |
| B | PRO450 |
| A | GLU75 |
| A | ASN132 |
| A | LEU134 |
| A | ASP208 |
| A | PRO210 |
| A | HIS211 |
| A | ARG219 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 1479 |
| Chain | Residue |
| A | ADP1478 |
| A | HOH2053 |
| A | HOH2063 |
| A | HOH2250 |
| A | HOH2251 |
| B | GLU16 |
| site_id | AC3 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE ADP B 1479 |
| Chain | Residue |
| A | GLY14 |
| A | ILE15 |
| A | GLU16 |
| A | HOH2016 |
| A | HOH2235 |
| A | HOH2236 |
| B | ARG60 |
| B | THR73 |
| B | ALA74 |
| B | GLU75 |
| B | ASN132 |
| B | LEU134 |
| B | PRO210 |
| B | HIS211 |
| B | ARG219 |
| B | ARG418 |
| B | TRP440 |
| B | PRO458 |
| B | MG1480 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 1480 |
| Chain | Residue |
| A | GLU16 |
| A | HOH2016 |
| A | HOH2017 |
| A | HOH2018 |
| A | HOH2235 |
| B | ADP1479 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:22910360 |
| Chain | Residue | Details |
| A | ASP64 | |
| B | ASP64 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_02111, ECO:0000269|PubMed:23601177 |
| Chain | Residue | Details |
| B | GLU16 | |
| B | ARG60 | |
| B | TYR62 | |
| B | GLU70 | |
| B | THR73 | |
| B | TRP440 | |
| A | GLU16 | |
| A | ARG60 | |
| A | TYR62 | |
| A | GLU70 | |
| A | THR73 | |
| A | TRP440 |
