3CX5

Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005750	cellular_component	mitochondrial respiratory chain complex III
A	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
A	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
A	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
A	0009060	biological_process	aerobic respiration
A	0016020	cellular_component	membrane
A	0045333	biological_process	cellular respiration
A	0046872	molecular_function	metal ion binding
A	0070469	cellular_component	respirasome
A	1902600	biological_process	proton transmembrane transport
B	0004222	molecular_function	metalloendopeptidase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005750	cellular_component	mitochondrial respiratory chain complex III
B	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
B	0006508	biological_process	proteolysis
B	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
B	0009060	biological_process	aerobic respiration
B	0016020	cellular_component	membrane
B	0030061	cellular_component	mitochondrial crista
B	0045333	biological_process	cellular respiration
B	0046872	molecular_function	metal ion binding
B	0070469	cellular_component	respirasome
B	1902600	biological_process	proton transmembrane transport
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005750	cellular_component	mitochondrial respiratory chain complex III
C	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
C	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
C	0009055	molecular_function	electron transfer activity
C	0009060	biological_process	aerobic respiration
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0022904	biological_process	respiratory electron transport chain
C	0045275	cellular_component	respiratory chain complex III
C	0045333	biological_process	cellular respiration
C	0046872	molecular_function	metal ion binding
C	0070469	cellular_component	respirasome
C	1902600	biological_process	proton transmembrane transport
D	0009055	molecular_function	electron transfer activity
D	0020037	molecular_function	heme binding
E	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
E	0016020	cellular_component	membrane
E	0051537	molecular_function	2 iron, 2 sulfur cluster binding
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005750	cellular_component	mitochondrial respiratory chain complex III
F	0005758	cellular_component	mitochondrial intermembrane space
F	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
F	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
F	0009060	biological_process	aerobic respiration
F	0016020	cellular_component	membrane
F	0034399	cellular_component	nuclear periphery
F	0045333	biological_process	cellular respiration
F	0070469	cellular_component	respirasome
F	1902600	biological_process	proton transmembrane transport
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005750	cellular_component	mitochondrial respiratory chain complex III
G	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
G	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
G	0009060	biological_process	aerobic respiration
G	0016020	cellular_component	membrane
G	0034551	biological_process	mitochondrial respiratory chain complex III assembly
G	0045333	biological_process	cellular respiration
G	0070469	cellular_component	respirasome
G	0099617	cellular_component	matrix side of mitochondrial inner membrane
G	1902600	biological_process	proton transmembrane transport
H	0005739	cellular_component	mitochondrion
H	0005743	cellular_component	mitochondrial inner membrane
H	0005750	cellular_component	mitochondrial respiratory chain complex III
H	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
H	0009060	biological_process	aerobic respiration
H	0016020	cellular_component	membrane
H	0045333	biological_process	cellular respiration
H	0070469	cellular_component	respirasome
I	0005739	cellular_component	mitochondrion
I	0005743	cellular_component	mitochondrial inner membrane
I	0005750	cellular_component	mitochondrial respiratory chain complex III
I	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
I	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
I	0009060	biological_process	aerobic respiration
I	0016020	cellular_component	membrane
I	0034551	biological_process	mitochondrial respiratory chain complex III assembly
I	0045333	biological_process	cellular respiration
I	0070469	cellular_component	respirasome
I	1902600	biological_process	proton transmembrane transport
L	0004222	molecular_function	metalloendopeptidase activity
L	0005515	molecular_function	protein binding
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0005750	cellular_component	mitochondrial respiratory chain complex III
L	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
L	0006627	biological_process	protein processing involved in protein targeting to mitochondrion
L	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
L	0009060	biological_process	aerobic respiration
L	0016020	cellular_component	membrane
L	0045333	biological_process	cellular respiration
L	0046872	molecular_function	metal ion binding
L	0070469	cellular_component	respirasome
L	1902600	biological_process	proton transmembrane transport
M	0004222	molecular_function	metalloendopeptidase activity
M	0005515	molecular_function	protein binding
M	0005739	cellular_component	mitochondrion
M	0005743	cellular_component	mitochondrial inner membrane
M	0005750	cellular_component	mitochondrial respiratory chain complex III
M	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
M	0006508	biological_process	proteolysis
M	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
M	0009060	biological_process	aerobic respiration
M	0016020	cellular_component	membrane
M	0030061	cellular_component	mitochondrial crista
M	0045333	biological_process	cellular respiration
M	0046872	molecular_function	metal ion binding
M	0070469	cellular_component	respirasome
M	1902600	biological_process	proton transmembrane transport
N	0005739	cellular_component	mitochondrion
N	0005743	cellular_component	mitochondrial inner membrane
N	0005750	cellular_component	mitochondrial respiratory chain complex III
N	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
N	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
N	0009055	molecular_function	electron transfer activity
N	0009060	biological_process	aerobic respiration
N	0016020	cellular_component	membrane
N	0016491	molecular_function	oxidoreductase activity
N	0022904	biological_process	respiratory electron transport chain
N	0045275	cellular_component	respiratory chain complex III
N	0045333	biological_process	cellular respiration
N	0046872	molecular_function	metal ion binding
N	0070469	cellular_component	respirasome
N	1902600	biological_process	proton transmembrane transport
O	0009055	molecular_function	electron transfer activity
O	0020037	molecular_function	heme binding
P	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
P	0016020	cellular_component	membrane
P	0051537	molecular_function	2 iron, 2 sulfur cluster binding
Q	0005739	cellular_component	mitochondrion
Q	0005743	cellular_component	mitochondrial inner membrane
Q	0005750	cellular_component	mitochondrial respiratory chain complex III
Q	0005758	cellular_component	mitochondrial intermembrane space
Q	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
Q	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
Q	0009060	biological_process	aerobic respiration
Q	0016020	cellular_component	membrane
Q	0034399	cellular_component	nuclear periphery
Q	0045333	biological_process	cellular respiration
Q	0070469	cellular_component	respirasome
Q	1902600	biological_process	proton transmembrane transport
R	0005739	cellular_component	mitochondrion
R	0005743	cellular_component	mitochondrial inner membrane
R	0005750	cellular_component	mitochondrial respiratory chain complex III
R	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
R	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
R	0009060	biological_process	aerobic respiration
R	0016020	cellular_component	membrane
R	0034551	biological_process	mitochondrial respiratory chain complex III assembly
R	0045333	biological_process	cellular respiration
R	0070469	cellular_component	respirasome
R	0099617	cellular_component	matrix side of mitochondrial inner membrane
R	1902600	biological_process	proton transmembrane transport
S	0005739	cellular_component	mitochondrion
S	0005743	cellular_component	mitochondrial inner membrane
S	0005750	cellular_component	mitochondrial respiratory chain complex III
S	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
S	0009060	biological_process	aerobic respiration
S	0016020	cellular_component	membrane
S	0045333	biological_process	cellular respiration
S	0070469	cellular_component	respirasome
T	0005739	cellular_component	mitochondrion
T	0005743	cellular_component	mitochondrial inner membrane
T	0005750	cellular_component	mitochondrial respiratory chain complex III
T	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
T	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
T	0009060	biological_process	aerobic respiration
T	0016020	cellular_component	membrane
T	0034551	biological_process	mitochondrial respiratory chain complex III assembly
T	0045333	biological_process	cellular respiration
T	0070469	cellular_component	respirasome
T	1902600	biological_process	proton transmembrane transport
W	0005515	molecular_function	protein binding
W	0005739	cellular_component	mitochondrion
W	0005758	cellular_component	mitochondrial intermembrane space
W	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
W	0006123	biological_process	mitochondrial electron transport, cytochrome c to oxygen
W	0009055	molecular_function	electron transfer activity
W	0020037	molecular_function	heme binding
W	0046872	molecular_function	metal ion binding
W	0070469	cellular_component	respirasome

Functional Information from PROSITE/UniProt

site_id	PS00143
Number of Residues	23
Details	INSULINASE Insulinase family, zinc-binding region signature. Ggsryatkd.GvAHLLNRFnFqNT

Chain	Residue	Details
B	GLY37-THR59

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: covalent => ECO:0000269|PubMed:11880631, ECO:0000269|PubMed:18390544, ECO:0007744|PDB:1KYO, ECO:0007744|PDB:3CX5

Chain	Residue	Details
W	CYS19
N	ILE365-LYS385
W	CYS22
C	GLY205-SER223
C	ASP309-LYS319
C	ILE365-LYS385
N	MET1-ASN27
N	TYR103-ARG110
N	GLY205-SER223
N	ASP309-LYS319

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:11880631, ECO:0000269|PubMed:18390544, ECO:0007744|PDB:1KYO, ECO:0007744|PDB:3CX5

Chain	Residue	Details
W	HIS23
N	GLY75-TYR102
N	VAL111-VAL135
N	ASN173-HIS204
N	TYR224-TYR246
N	LYS288-THR308
N	VAL320-GLY340
N	TYR348-LEU364
W	MET85
C	VAL111-VAL135
C	ASN173-HIS204
C	TYR224-TYR246
C	LYS288-THR308
C	VAL320-GLY340
C	TYR348-LEU364
N	TYR28-ALA51

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6,N6,N6-trimethyllysine; by CTM1 => ECO:0000269|PubMed:10791961, ECO:0000269|PubMed:11880631, ECO:0007744|PDB:1KYO

Chain	Residue	Details
W	M3L77
T	ASN44-GLU66
C	SER247-ASP287
C	ALA341-PRO347
N	MET52-ASN74
N	TYR136-SER172
N	SER247-ASP287
N	ALA341-PRO347

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6,N6,N6-trimethyllysine => ECO:0000269|PubMed:10821864, ECO:0000269|PubMed:18390544

Chain	Residue	Details
W	LYS78
E	HIS161
E	CYS178
E	HIS181
P	CYS159
P	HIS161
P	CYS178
P	HIS181

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:11880631, ECO:0000269|PubMed:18390544, ECO:0000269|PubMed:30598554, ECO:0007744|PDB:1KYO

Chain	Residue	Details
D	HIS105
O	HIS105
C	HIS183
C	HIS197
N	HIS82
N	HIS96
N	HIS183
N	HIS197

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:10873857, ECO:0000269|PubMed:11880631, ECO:0000269|PubMed:18390544, ECO:0000269|PubMed:30598554, ECO:0007744|PDB:1EZV, ECO:0007744|PDB:1KYO

Chain	Residue	Details
D	MET225
O	MET225

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 208

Chain	Residue	Details
E	HIS181	covalently attached, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser
C	SER206	electrostatic stabiliser, hydrogen bond donor, radical stabiliser
C	LYS228	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser
C	ASP229	electrostatic stabiliser, hydrogen bond acceptor, radical stabiliser
C	GLU272	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser

---

site_id	MCSA2
Number of Residues	1
Details	M-CSA 208

Chain	Residue	Details
P	HIS181	covalently attached, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser
N	SER206	electrostatic stabiliser, hydrogen bond donor, radical stabiliser
N	LYS228	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser
N	ASP229	electrostatic stabiliser, hydrogen bond acceptor, radical stabiliser
N	GLU272	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, radical stabiliser

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