2OKK

The X-ray crystal structure of the 65kDa isoform of Glutamic Acid Decarboxylase (GAD65)

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Functional Information from GO Data

ChainGOidnamespacecontents
A0031225cellular_componentanchored component of membrane
A0030424cellular_componentaxon
A0030054cellular_componentcell junction
A0061202cellular_componentclathrin-sculpted gamma-aminobutyric acid transport vesicle membrane
A0005829cellular_componentcytosol
A0000139cellular_componentGolgi membrane
A0060077cellular_componentinhibitory synapse
A0048471cellular_componentperinuclear region of cytoplasm
A0005886cellular_componentplasma membrane
A0042734cellular_componentpresynaptic membrane
A0030672cellular_componentsynaptic vesicle membrane
A0016595molecular_functionglutamate binding
A0004351molecular_functionglutamate decarboxylase activity
A0046982molecular_functionprotein heterodimerization activity
A0030170molecular_functionpyridoxal phosphate binding
A0007268biological_processchemical synaptic transmission
A0006540biological_processglutamate decarboxylation to succinate
A0042136biological_processneurotransmitter biosynthetic process
A0042493biological_processresponse to drug
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
AC16BINDING SITE FOR RESIDUE ABU A 585
ChainResidue
AGLN181
ALEU182
ASER183
APHE205
ATHR339
AARG558

AC28BINDING SITE FOR RESIDUE ABU A 586
ChainResidue
AGLN181
ALEU182
ASER183
AASN203
AHIS282
ALLP396
ACYS446
AHOH661

AC35BINDING SITE FOR RESIDUE GOL A 587
ChainResidue
AGLU279
ASER281
AHIS282
APHE283
ALYS286

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
ABU_2okk_A_5858GAMMA-AMINO-BUTANOIC ACID binding site
ChainResidueligand
AASN180-SER183ABU: GAMMA-AMINO-BUTANOIC ACID
ATHR339ABU: GAMMA-AMINO-BUTANOIC ACID
ALLP396ABU: GAMMA-AMINO-BUTANOIC ACID
ASER546ABU: GAMMA-AMINO-BUTANOIC ACID
AARG558ABU: GAMMA-AMINO-BUTANOIC ACID

ABU_2okk_A_5868GAMMA-AMINO-BUTANOIC ACID binding site
ChainResidueligand
AGLN181-SER183ABU: GAMMA-AMINO-BUTANOIC ACID
AHIS282-PHE283ABU: GAMMA-AMINO-BUTANOIC ACID
ATHR339ABU: GAMMA-AMINO-BUTANOIC ACID
AHIS395-LLP396ABU: GAMMA-AMINO-BUTANOIC ACID

GOL_2okk_A_58710GLYCEROL binding site
ChainResidueligand
ATHR277-PHE283GOL: GLYCEROL
ALEU285-LYS286GOL: GLYCEROL
ALEU301GOL: GLYCEROL

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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
PS0039222DDC / GAD / HDC / TyrDC pyridoxal-phosphate attachment site. S-[LIVMFYW]-x-{KG}-x(3)-K-[LIVMFYWGH]-[LIVMFYWG]-x-{R}-x-[LIVMFYW]-{V}
ChainResidueDetails
ASER389-ARG410

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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
SWS_FT_FI11Substrate.
ChainResidueDetails
AARG471

CSA12Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AASP364
AHIS282

CSA22Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AHIS280
AASP364

CSA32Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AASP364
APHE283

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Catalytic Information from CSA

site_idNumber of ResiduesDetails
CSA12Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AASP364
AHIS282

CSA22Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AHIS280
AASP364

CSA32Annotated By Reference To The Literature 1ecx
ChainResidueDetails
AASP364
APHE283