1YSB

Yeast Cytosine Deaminase Triple Mutant

GO（遺伝子オントロジー）由来の情報

鎖名	GO（遺伝子オントロジー）id	名前空間	内容
A	0003824	molecular_function	catalytic activity
A	0004131	molecular_function	cytosine deaminase activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0006139	biological_process	nucleobase-containing compound metabolic process
A	0008270	molecular_function	zinc ion binding
A	0008655	biological_process	pyrimidine-containing compound salvage
A	0008835	molecular_function	diaminohydroxyphosphoribosylaminopyrimidine deaminase activity
A	0016787	molecular_function	hydrolase activity
A	0016814	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
A	0019239	molecular_function	deaminase activity
A	0019858	biological_process	cytosine metabolic process
A	0044206	biological_process	UMP salvage
A	0046087	biological_process	cytidine metabolic process
A	0046872	molecular_function	metal ion binding
A	0102480	molecular_function	5-fluorocytosine deaminase activity
B	0003824	molecular_function	catalytic activity
B	0004131	molecular_function	cytosine deaminase activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0008270	molecular_function	zinc ion binding
B	0008655	biological_process	pyrimidine-containing compound salvage
B	0008835	molecular_function	diaminohydroxyphosphoribosylaminopyrimidine deaminase activity
B	0016787	molecular_function	hydrolase activity
B	0016814	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
B	0019239	molecular_function	deaminase activity
B	0019858	biological_process	cytosine metabolic process
B	0044206	biological_process	UMP salvage
B	0046087	biological_process	cytidine metabolic process
B	0046872	molecular_function	metal ion binding
B	0102480	molecular_function	5-fluorocytosine deaminase activity

PDBデータベースに由来する情報

site_id	AC1
残基数	5
詳細	BINDING SITE FOR RESIDUE ZN B 500

鎖名	残基
B	HIS262
B	CYS291
B	CYS294
B	ZN502
B	HOH1332

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site_id	AC2
残基数	5
詳細	BINDING SITE FOR RESIDUE ZN A 501

鎖名	残基
A	HOH1335
A	HIS62
A	CYS91
A	CYS94
A	ZN503

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site_id	AC3
残基数	6
詳細	BINDING SITE FOR RESIDUE ZN B 502

鎖名	残基
B	GLU264
B	ZN500
B	HOH1054
B	HOH1331
B	HOH1332
B	HOH1333

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site_id	AC4
残基数	6
詳細	BINDING SITE FOR RESIDUE ZN A 503

鎖名	残基
A	GLU64
A	ZN501
A	HOH1044
A	HOH1334
A	HOH1335
A	HOH1336

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site_id	AC5
残基数	5
詳細	BINDING SITE FOR RESIDUE CA B 504

鎖名	残基
B	GLY326
B	ASP346
B	HOH1026
B	HOH1065
B	HOH1071

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UniProtにおけるモチーフ・データベースPROSITEからの機能情報

site_id	PS00903
残基数	37
詳細	CYT_DCMP_DEAMINASES_1 Cytidine and deoxycytidylate deaminases zinc-binding region signature. HGEisTLencgrlegkvykdttlyttls............PCdm......CtgaI

鎖名	残基	詳細
A	HIS62-ILE98

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SwissProt/UniProtに記載されている蛋白質分子機能情報

site_id	SWS_FT_FI1
残基数	2
詳細	ACT_SITE: Proton donor => ECO:0000269|PubMed:12637534, ECO:0007744|PDB:1UAQ

鎖名	残基	詳細
A	GLU64
B	GLU264

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site_id	SWS_FT_FI2
残基数	4
詳細	BINDING: BINDING => ECO:0000269|PubMed:12637534, ECO:0000269|PubMed:12906827, ECO:0007744|PDB:1P6O, ECO:0007744|PDB:1UAQ

鎖名	残基	詳細
A	ASN51
A	ASP155
B	ASN251
B	ASP355

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site_id	SWS_FT_FI3
残基数	6
詳細	BINDING: BINDING => ECO:0000269|PubMed:12637534, ECO:0000269|PubMed:12906827, ECO:0000269|PubMed:15879217, ECO:0007744|PDB:1OX7, ECO:0007744|PDB:1P6O, ECO:0007744|PDB:1RB7, ECO:0007744|PDB:1UAQ, ECO:0007744|PDB:1YSB, ECO:0007744|PDB:1YSD

鎖名	残基	詳細
B	CYS291
B	CYS294
A	HIS62
A	CYS91
A	CYS94
B	HIS262

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CSAにおける酵素触媒機能の情報

site_id	MCSA1
残基数	5
詳細	M-CSA 636

鎖名	残基	詳細
A	HIS62	metal ligand
A	GLU64	proton acceptor, proton donor
A	SER89	electrostatic stabiliser
A	CYS94	metal ligand
A	CYS91	metal ligand

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site_id	MCSA2
残基数	5
詳細	M-CSA 636

鎖名	残基	詳細
B	HIS262	metal ligand
B	GLU264	proton acceptor, proton donor
B	SER289	electrostatic stabiliser
B	CYS294	metal ligand
B	CYS291	metal ligand

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