1RR8

Structural Mechanisms of Camptothecin Resistance by Mutations in Human Topoisomerase I

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0003677	molecular_function	DNA binding
C	0003917	molecular_function	DNA topoisomerase type I (single strand cut, ATP-independent) activity
C	0005694	cellular_component	chromosome
C	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	1
Number of Residues
Details

Chain

Residue

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site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE TTG A 1991

Chain	Residue
A	DT10
C	LYS532
C	ASP533
C	THR718
C	ASN722
A	DG11
A	DG12
A	TTC100
A	HOH886
B	DC112
B	DA113
C	GLU356
C	ARG364

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site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE TTC A 100

Chain	Residue
A	DT10
A	DG11
A	DG12
A	HOH886
A	TTG1991
B	DC112
B	DA113
B	HOH878
C	GLU356
C	ARG364
C	LYS532
C	ASP533
C	THR718
C	PTR723

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Functional Information from PROSITE/UniProt

site_id	PS00176
Number of Residues	19
Details	TOPO_IB_1 Topoisomerase (Topo) IB-type active site signature. EnkqialGTSKlnYLdprI

Chain	Residue	Details
C	GLU710-ILE728

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: O-(3'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01382, ECO:0000255|PROSITE-ProRule:PRU10130, ECO:0000269|PubMed:10841763, ECO:0000269|PubMed:12426403, ECO:0000269|PubMed:12533542, ECO:0000269|PubMed:15165849, ECO:0000269|PubMed:15801827, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:9488644

Chain	Residue	Details
C	PTR723

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site_id	SWS_FT_FI2
Number of Residues	9
Details	SITE: Interaction with DNA

Chain	Residue	Details
C	ARG316
C	ARG364
C	TRP412
C	LYS443
C	THR501
C	LYS532
C	ASN574
C	HIS632
C	LYS650

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
C	LYS280

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CK2 => ECO:0000269|PubMed:23185622

Chain	Residue	Details
C	SER506

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site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
C	LYS204
C	LYS549
C	LYS642
C	LYS700
C	LYS712

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site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
C	LYS336

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