1E3X
Native structure of chimaeric amylase from B. amyloliquefaciens and B. licheniformis at 1.92A
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004556 | molecular_function | alpha-amylase activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005615 | cellular_component | extracellular space |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008152 | biological_process | metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 501 |
| Chain | Residue |
| A | ASN102 |
| A | ASP194 |
| A | ASP200 |
| A | HIS235 |
| A | HOH2204 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 502 |
| Chain | Residue |
| A | ASP204 |
| A | HOH2367 |
| A | ASP159 |
| A | ALA181 |
| A | ASP183 |
| A | ASP202 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CA A 503 |
| Chain | Residue |
| A | ASN444 |
| A | GLU447 |
| A | HOH2615 |
| A | HOH2618 |
| A | HOH2619 |
| A | HOH2635 |
| A | HOH2679 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 504 |
| Chain | Residue |
| A | GLY300 |
| A | TYR302 |
| A | HIS406 |
| A | ASP407 |
| A | ASP430 |
| A | HOH2488 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 505 |
| Chain | Residue |
| A | ASP159 |
| A | ASP183 |
| A | ASP194 |
| A | ASP200 |
| A | VAL201 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| A | ASP231 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | GLU261 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10924103, ECO:0000269|PubMed:20124706, ECO:0007744|PDB:1E40 |
| Chain | Residue | Details |
| A | ASN102 | |
| A | ALA181 | |
| A | ASP202 | |
| A | ASP204 | |
| A | HIS235 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10924103, ECO:0007744|PDB:1E40 |
| Chain | Residue | Details |
| A | ASP159 | |
| A | ASP183 | |
| A | ASP194 | |
| A | ASP200 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20124706, ECO:0007744|PDB:3BH4 |
| Chain | Residue | Details |
| A | GLY300 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12540849, ECO:0000269|PubMed:9551551, ECO:0007744|PDB:1BLI, ECO:0007744|PDB:1OB0 |
| Chain | Residue | Details |
| A | ASP407 | |
| A | ASP430 | |
| A | TYR302 | |
| A | HIS406 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | SITE: Transition state stabilizer => ECO:0000250 |
| Chain | Residue | Details |
| A | ASP328 |
