9JQG

Crystal structure of rice DWARF14 in complex with Cyclo(L-Leu-L-Pro)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	48.093, 88.560, 119.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 1.500
R-factor	0.1698
Rwork	0.169
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.065
Number of reflections	81350	3893
<I/σ(I)>	15.8	2.4
Completeness [%]	99.7
Redundancy	5.5
CC(1/2)		0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.16	1,6-Hexanediol;1-Butanol; 1,2-Propanediol (racemic); 2-Propanol;1,4 Butanediol;1,3-Propanediol ;Imidazole;MES;Glycerol;PEG 4000