9H6A

Crystal structure of the E. coli F-plasmid VapBC toxin-antitoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.984010
Spacegroup name	P 32 2 1
Unit cell lengths	90.540, 90.540, 119.889
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.620 - 2.860
R-factor	0.2308
Rwork	0.225
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.708
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.620	47.620	2.930
High resolution limit [Å]	2.860	12.790	2.860
Rmerge	0.149	0.028	4.626
Rmeas	0.153	0.029	4.747
Number of reflections	13589	181	981
<I/σ(I)>	16.71
Completeness [%]	100.0
Redundancy	20
CC(1/2)	1.000	1.000	0.224

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Lithium sulphate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 26 % PEG200