Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000096 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.353, 90.353, 82.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.510 - 1.740 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.742 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.018 | 1.826 |
| High resolution limit [Å] | 1.731 | 1.731 |
| Rpim | 0.013 | 0.451 |
| Number of reflections | 31365 | 1568 |
| <I/σ(I)> | 23.2 | 1.4 |
| Completeness [%] | 95.6 | |
| Redundancy | 13.4 | |
| CC(1/2) | 1.000 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2 M ammonium sulfate, 5 % (v/v) PEG 400, 0.1 M MES pH 6.5 |
