9HKG
X-ray structure of Perm2, a circularly permuted mutant of the sweet protein MNEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 39.464, 32.518, 40.824 |
| Unit cell angles | 90.00, 97.84, 90.00 |
Refinement procedure
| Resolution | 39.100 - 1.260 |
| R-factor | 0.1598 |
| Rwork | 0.159 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.264 |
| Rmerge | 0.066 | 0.887 |
| Rmeas | 0.072 | |
| Rpim | 0.029 | 0.494 |
| Number of reflections | 23712 | 1035 |
| <I/σ(I)> | 14.46 | 1.05 |
| Completeness [%] | 85.6 | 37.35 |
| Redundancy | 6.3 | 3.8 |
| CC(1/2) | 0.993 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Precipitant: 19% (w/v) PEG 4000, 19% (v/v) 2-Propanol Buffer: 0.095 M Trisodium citrate (pH 5.5) Additive: 5% (v/v) Glycerol |
