9H7Q

MITF in complex with 5-chloro-3-phenyl-1H-indole-2-carboxylic acid

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999955
Spacegroup name	P 41 21 2
Unit cell lengths	90.535, 90.535, 82.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.020 - 1.720
R-factor	0.2161
Rwork	0.214
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.511
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.020	1.750
High resolution limit [Å]	1.720	1.720
Rpim	0.014	1.199
Number of reflections	37356	1832
<I/σ(I)>	19	0.5
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	1.000	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.2 M ammonium sulfate, 5 % (v/v) PEG 400, 0.1 M MES pH 6.5