9H44
Jumonji domain-containing protein 2B with crown ether and crystallization epitope mutations L916G:R917A:A918D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.930, 87.930, 142.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.308 - 1.740 |
| Rwork | 0.203 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.048 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.82)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.310 | 1.790 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.053 | 0.746 |
| Number of reflections | 21921 | 1522 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Hampton Index well B7 0.056 M sodium phosphate monobasic 1.344 M potassium phosphate dibasic |
