9H16
Crystal structure of OXA-405 apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-07 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 122.081, 122.081, 159.119 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.040 - 1.560 |
| R-factor | 0.1756 |
| Rwork | 0.175 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.841 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.570 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rpim | 0.053 | 1.629 |
| Number of reflections | 99172 | 9689 |
| <I/σ(I)> | 8 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 13.3 |
| CC(1/2) | 0.997 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.1 M Tris pH 8.5, 28% PEG 550 |
