9DGW
X-ray crystal structure of the Viperin-like enzyme from T. virens with bound CTP and SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.569, 80.309, 105.613 |
| Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
| Resolution | 28.590 - 1.720 |
| R-factor | 0.26284 |
| Rwork | 0.261 |
| R-free | 0.29231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.590 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.070 | 0.445 |
| Rmeas | 0.095 | 0.587 |
| Rpim | 0.063 | 0.377 |
| Number of reflections | 354684 | 17788 |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 99.3 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.992 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M Bis-Tris pH 6.5, 0.2 M NaCl, 25% (w/v) PEG 3350 |
