8YAU

Crystal structure of glucose 1-dehydrogenase mutant2 from Limosilactobacillus fermentum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.988
Spacegroup name	C 2 2 21
Unit cell lengths	61.749, 139.523, 331.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.560 - 2.220
R-factor	0.2395
Rwork	0.238
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.220	2.220
Number of reflections	71146	10230
<I/σ(I)>	19.3	2.6
Completeness [%]	100.0
Redundancy	26.3
CC(1/2)	0.999	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M MMT pH 6.0, 25% PEG 1500