8ZGB

W-degron fused ZZ-domain of the Arabidopsis thaliana E3 ubiquitin-protein ligase PRT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28
Spacegroup name	I 41 2 2
Unit cell lengths	106.260, 106.260, 97.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.770 - 2.785
R-factor	0.2373
Rwork	0.234
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zg8
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.770	2.884
High resolution limit [Å]	2.785	2.785
Rmerge	0.126	2.093
Rmeas	0.129	2.131
Rpim	0.024	0.392
Number of reflections	7204	683
<I/σ(I)>	18.48	1.02
Completeness [%]	99.6
Redundancy	28.8
CC(1/2)	0.997	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 400 Tris base/ Hydrochloric acid pH 8.5 Lithium sulfate